Meiri H, Meiri U, Kennedy D R, Marshall J M
Am J Physiol. 1978 Mar;234(3):C96-101. doi: 10.1152/ajpcell.1978.234.3.C96.
This study attempts to distinguish between a direct action of ovarian steroids on adrenergic neurons in the oviduct and an indirect effect mediated by changes in muscle size. Mature rabbits were treated as follows: group 1, ovariectomized and a polyethylene catheter (1 mm OD) inserted into isthmus of one oviduct (CT) with contralateral oviduct as control (C); group 2, normal, estrous animals with one intubated isthmus (ET) and contralateral control (E). Fourteen days postoperatively, oviducts were removed and muscle-wall thickness, norepinephrine (NE) content, and response to nerve stimulation were measured. Although castration atrophy was prevented in CT, NE content of CT was significantly less than C, 0.032 +/- 0.07 versus 1.09 +/- 0.10 nmol. NE content of ET was also significantly less than E (1.32 +/- 0.03 versus 1.81 +/- 2.0) despite a greater wall thickness of ET. It was concluded that: a) withdrawal of ovarian hormones reduces NE contents by a direct action on nerves; b) moderate stretch, per se, increases muscle size and reduces NE content; c) nerve stimulation induces muscle contraction despite large reduction in transmitter content.
本研究试图区分卵巢甾体激素对输卵管肾上腺素能神经元的直接作用以及由肌肉大小变化介导的间接作用。成年兔的处理如下：第1组，切除卵巢并将一根外径1毫米的聚乙烯导管插入一侧输卵管峡部（CT），对侧输卵管作为对照（C）；第2组，处于发情期的正常动物，一侧输卵管峡部插管（ET），对侧作为对照（E）。术后14天，取出输卵管并测量肌壁厚度、去甲肾上腺素（NE）含量以及对神经刺激的反应。尽管CT组预防了去势萎缩，但CT组的NE含量显著低于C组，分别为0.032±0.07与1.09±0.10纳摩尔。尽管ET组的肌壁厚度更大，但其NE含量也显著低于E组（1.32±0.03与1.81±2.0）。得出的结论是：a）卵巢激素的撤除通过对神经的直接作用降低NE含量；b）适度拉伸本身会增加肌肉大小并降低NE含量；c）尽管递质含量大幅降低，但神经刺激仍可诱导肌肉收缩。
