Breckenridge W C, Kuksis A
J Lipid Res. 1968 May;9(3):388-93.
Triglycerides with a total of 34-44 acyl carbon atoms have previously been shown to account for nearly 50% of bovine milk fat. About 80% of this material was isolated by molecular distillation and a representative fraction of these glycerides was subjected to a stereospecific analysis. Of the 29 moles % of C(4)-C(8) fatty acids present in the analyzed fraction, at least 95% were specifically attached to the glycerol molecule in the position corresponding to carbon 3 of sn-glycerol 3-(dihydrogen phosphate) (L-alpha-glycerophosphate). The distribution of the other fatty acids (C(10) or greater) did not show such marked specificity for either the 1- or the 2-position. The data support the hypothesis that the short-chain fatty acids are esterified with long-chain diglycerides, or are substituted in glycerophosphatide intermediates, during the final step in the biosynthesis of milk fat triglycerides.
先前的研究表明，总共有34 - 44个酰基碳原子的甘油三酯占牛乳脂肪的近50%。其中约80%的这种物质通过分子蒸馏分离出来，并且对这些甘油酯的一个代表性部分进行了立体专一性分析。在所分析的部分中存在的29摩尔%的C(4)-C(8)脂肪酸中，至少95%特异性地连接在甘油分子上，其位置对应于sn-甘油3-(磷酸二氢酯)(L-α-甘油磷酸酯)的甘油碳3位。其他脂肪酸（C(10)或更长链）的分布对于1-位或2-位均未显示出如此明显的特异性。这些数据支持这样的假说：在乳脂肪甘油三酯生物合成的最后一步中，短链脂肪酸与长链甘油二酯酯化，或在甘油磷脂中间体中被取代。
