Brookes V J, Dejmal R K
Science. 1968 May 31;160(3831):999-1001. doi: 10.1126/science.160.3831.999-a.
Most of the yolk protein in the mature egg of Leucophaea maderae consists of one large component, whereas a second smaller protein is present during the early stages of vitellogenesis. The large protein can be converted to the smaller one and to even smaller units by mild alkaline conditions in vitro. After injection of uniformly labeled leucine-C(14) into females with developing eggs, the smaller yolk protein becomes labeled first, the label is then transferred to the large protein upon prolonged exposure.
麻蝇成熟卵中的大部分卵黄蛋白由一种大分子成分组成,而在卵黄生成早期存在第二种较小的蛋白质。在体外温和的碱性条件下,大分子蛋白质可转化为较小的蛋白质,甚至更小的单位。在用放射性同位素均匀标记亮氨酸-C(14)注射到有发育中卵的雌蝇体内后,较小的卵黄蛋白首先被标记,经过较长时间后,标记转移到大分子蛋白质上。