Uhlenbruck G, Baldo B A, Steinhausen G, Schwick H G, Chatterjee B P, Horejsí V, Krajhanzl A, Kocourek J
J Clin Chem Clin Biochem. 1978 Jan;16(1):19-23. doi: 10.1515/cclm.1978.16.1.19.
Highly purified human serum glycoproteins were treated with neuraminidase and examined for their cross reaction with several lectins with anti-galactosyl specificity: beta-D-galactosyl structures are thought to be the main terminal sugar residues that become attached de novo after removal of neuraminic acid. The following lectins were tested: Tridacnin from the bivalve clams Tridacna maxima and Tridacna gigas, the agglutinin from the sponge Axinella polypoides, the lectin from the roach Rutilus rutilus and the plant lectins from Ricinus communis, Ononis spinosa, Glycine soja and Abrus precatorius. In agar gel diffusion, these purified and precipitating lectins gave more or less strong or negative results against the different neuraminidase-treated serum glycoproteins, thus indicating subtle differences with respect to their anti-galactosyl combining specificity. On the other hand, serum glycoproteins which reacted with the same lectin, did not always show complete identity lines. Finally, as revealed by these lectins, the carbohydrate moiety of serum glycoproteins may reflect a complex and broad spectrum of heterogeneity. This could lead to a more detailed understanding of the topographical and steric arrangement of the chemical structure and of the biological role of carbohydrate groups in these glycosubstances.
用神经氨酸酶处理高度纯化的人血清糖蛋白，并检测它们与几种具有抗半乳糖基特异性的凝集素的交叉反应：β-D-半乳糖基结构被认为是去除神经氨酸后重新连接的主要末端糖残基。测试了以下凝集素：来自大砗磲和长砗磲的双壳贝类的砗磲凝集素、来自多孔海绵的凝集素、来自拟鲤的凝集素以及来自蓖麻、棘豆、大豆和相思子的植物凝集素。在琼脂凝胶扩散试验中，这些纯化的沉淀凝集素对不同的经神经氨酸酶处理的血清糖蛋白给出了或多或少的强阳性或阴性结果，从而表明它们在抗半乳糖基结合特异性方面存在细微差异。另一方面，与同一凝集素反应的血清糖蛋白并不总是显示出完全相同的沉淀线。最后，通过这些凝集素揭示，血清糖蛋白的碳水化合物部分可能反映出复杂且广泛的异质性。这可能会导致对这些糖物质中化学结构的拓扑和空间排列以及碳水化合物基团的生物学作用有更详细的了解。
