Schottelius J, Marinkelle C J, Gomez-Leiva M A
Trop Med Parasitol. 1986 Mar;37(1):54-8.
Four-days old epimastigote culture forms of different stocks of Trypanosoma cruzi from Colombia, Costa Rica and Mexico as well of Colombian stocks of T. rangeli were tested with 27 lectins and the complement lysis test. While the stocks of T. cruzi and T. rangeli showed common agglutination reactions with the lectins of Canavalia ensiformis and Pisum sativum, only the stocks of T. cruzi were additionally agglutinated by Ricinus communis-120, Glycine maxima, Helix pomatia, Axinella polypoides, Bandeiraea simplicifolia, Bauhinia purpurea, Wistaria floribunda, Abrus precatorius, Aaptos papillata II, Limax flavus and Arachis hypogaea. On the basis of lectin typing, the strains of T. cruzi belong to the PNA-type. While the epimastigote culture forms of T. cruzi stocks were lysed by normal fresh human, rat, chicken, rabbit and guinea pig serum, the culture forms of T. rangeli were only lysed by chicken serum. Mouse serum had no lytic effect on either trypanosome species. Incubation of T. cruzi with neuraminidase did not alter the lytic effect of the sera. The membrane-associated N-acetylneuraminic acid on T. cruzi does not protect the cell surface against the activation of the alternative complement pathway. After treatment of T. cruzi with neuraminidase and subsequent incubation in culture medium, the PNA-type was restored. The cells reacted again with the lectins of Arachis hypogaea, Limax flavus, Aaptos papillata II but not with Triticum vulgaris.
用27种凝集素和补体溶血试验对来自哥伦比亚、哥斯达黎加和墨西哥的不同克氏锥虫株以及哥伦比亚的兰氏锥虫株的4日龄上鞭毛体培养形式进行了检测。虽然克氏锥虫株和兰氏锥虫株与刀豆和豌豆的凝集素表现出共同的凝集反应，但只有克氏锥虫株还被蓖麻-120、大豆、玉黍螺、多孔轴海绵、单叶相思、紫羊蹄甲、紫藤、相思子、乳头艾普托海绵II、黄斑蛞蝓和落花生凝集。基于凝集素分型，克氏锥虫株属于PNA型。虽然克氏锥虫株的上鞭毛体培养形式可被正常新鲜人、大鼠、鸡、兔和豚鼠血清溶解，但兰氏锥虫的培养形式仅被鸡血清溶解。小鼠血清对这两种锥虫均无溶解作用。用神经氨酸酶处理克氏锥虫不会改变血清的溶解作用。克氏锥虫表面与膜相关的N-乙酰神经氨酸不能保护细胞表面免受替代补体途径的激活。用神经氨酸酶处理克氏锥虫并随后在培养基中孵育后，PNA型得以恢复。细胞再次与落花生、黄斑蛞蝓、乳头艾普托海绵II的凝集素发生反应，但不与普通小麦的凝集素反应。
