Altenbern R A
J Bacteriol. 1968 May;95(5):1642-6. doi: 10.1128/jb.95.5.1642-1646.1968.
The genome of Staphylococcus aureus was mapped by enumerating mutants induced by nitrosoguanidine during synchronous chromosomal replication following release from phenethanol inhibition. Both chromosomal replication time and cell division time were 120 min for this strain of S. aureus. Duplication of genes occurred within a 10-min period of the 120 min required for chromosomal replication. A high-resolution method was devised to determine the gene order of four genes that duplicated in the same 10-min interval of replication of the chromosome. A genomic map locating the positions of 10 genes was derived.
通过对亚硝基胍诱导的突变体进行计数,绘制了金黄色葡萄球菌在从苯乙醇抑制释放后同步染色体复制期间的基因组图谱。该金黄色葡萄球菌菌株的染色体复制时间和细胞分裂时间均为120分钟。基因复制发生在染色体复制所需的120分钟中的10分钟时间段内。设计了一种高分辨率方法来确定在染色体复制的相同10分钟间隔内复制的四个基因的基因顺序。得出了一张定位10个基因位置的基因组图谱。