通过在粪链球菌同步群体中诱导突变进行染色体图谱绘制。
Chromosomal mapping by means of mutational induction in synchronous populations of Streptococcus faecalis.
作者信息
Stonehill E H, Hutchison D J
出版信息
J Bacteriol. 1966 Jul;92(1):136-43. doi: 10.1128/jb.92.1.136-143.1966.
Stonehill, E. H. (Sloan-Kettering Institute for Cancer Research, New York, N.Y. and Dorris J. Hutchison. Chromosomal mapping by means of mutational induction in synchronous populations of Streptococcus faecalis. J. Bacteriol. 92:136-143. 1966.-A new method for mapping genetic loci on the bacterial chromosome, based on the linear progression of the deoxyribonucleic acid-replicating enzyme (replicase) in synchronous cultures of Streptococcus faecalis, is described. Ultraviolet irradiation was used to induce mutations to drug and to bacteriophage resistance at various intervals during the progress of the replicase along the chromosome. The time of duplication of the gene in synchronous populations was determined from reductions in the rates of irradiation-induced mutation. After the replication of various genes, the rates fell to half their former values. The resulting data made possible the construction of a 1-hr time-map indicating the sequence of replication of five genes in S. faecalis.
斯通希尔，E. H.（纽约市斯隆 - 凯特琳癌症研究所，以及多里斯·J. 哈钦森）。通过粪肠球菌同步群体中的突变诱导进行染色体定位。《细菌学杂志》92:136 - 143，1966年。——描述了一种基于粪肠球菌同步培养物中脱氧核糖核酸复制酶（复制酶）的线性进展来绘制细菌染色体上基因位点的新方法。在复制酶沿着染色体移动的不同时间间隔，用紫外线照射诱导对药物和噬菌体的抗性突变。从照射诱导突变率的降低来确定同步群体中基因复制的时间。在各种基因复制后，突变率降至先前值的一半。所得数据使得构建一个1小时时间图谱成为可能，该图谱显示了粪肠球菌中五个基因的复制顺序。
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