DeMoss R D
J Bacteriol. 1968 May;95(5):1692-7. doi: 10.1128/jb.95.5.1692-1697.1968.
Enzyme extracts of Leuconostoc mesenteroides were found to contain at least four separate kinases: one active with glucose, glucosamine, and N-acetylglucosamine; one active with fructose and mannose; and two active with gluconate, one constitutive and one inducible. The molecular sizes of all the kinases, estimated from sucrose gradient centrifugation, are about the same, 37,000 to 50,000 daltons, except the constitutive gluconate kinase, which is about 100,000 daltons. Apparent Michaelis constants were calculated for all of the substrates mentioned. The kinases are separable on triethylaminoethyl cellulose.
一种对葡萄糖、氨基葡萄糖和N - 乙酰氨基葡萄糖有活性;一种对果糖和甘露糖有活性;还有两种对葡萄糖酸盐有活性,一种是组成型的,一种是诱导型的。通过蔗糖梯度离心法估算,所有激酶的分子大小大致相同,为37,000至50,000道尔顿,但组成型葡萄糖酸盐激酶约为100,000道尔顿。计算了上述所有底物的表观米氏常数。这些激酶在三乙氨基乙基纤维素上是可分离的。