维涅兰德固氮菌OP及一个明显部分组成型突变体中固氮作用的调控
Regulation of nitrogen fixation in Azotobacter vinelandii OP and in an apparently partially constitutive mutant.
作者信息
Sorger G J
出版信息
J Bacteriol. 1968 May;95(5):1721-6. doi: 10.1128/jb.95.5.1721-1726.1968.
Abstract
Methylamine and 2-methylalanine appeared to act as co-repressors of nitrogenase in Azotobacter vinelandii OP. They inhibited the growth of this organism on molecular nitrogen but not on nitrate, ammonia, or Casamino Acids; they prevented the formation of nitrogenase by cells transferred from repression to induction conditions; and they did not inhibit the activity of nitrogenase in vitro. A mutant of strain OP, selected on the basis of its relative resistance to methylalanine, appeared partially constitutive because nitrogenase in this strain was less sensitive to repressors than was the enzyme in the wild-type strain.
摘要
甲胺和2-甲基丙氨酸似乎作为棕色固氮菌OP中固氮酶的共阻遏物。它们抑制该生物体在分子氮上的生长，但不抑制在硝酸盐、氨或酪蛋白氨基酸上的生长；它们阻止从阻遏条件转移到诱导条件的细胞形成固氮酶；并且它们在体外不抑制固氮酶的活性。基于其对甲基丙氨酸的相对抗性选择的OP菌株的一个突变体似乎部分组成型，因为该菌株中的固氮酶比野生型菌株中的酶对阻遏物更不敏感。
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