Kurz W G, LaRue T A, Chatson K B
Can J Microbiol. 1975 Jul;21(7):984-8. doi: 10.1139/m75-145.
Azotobacter vinelandii OP was synchronized by the continuous phased culture technique. The nitrogenase (nitrogen:(acceptor)oxidoreductase)(EC 1.7.99.2) activity of the culture was determined continuously within the fermentor by acetylene reduction. Addition of NH4+ in excess of 5 x 10(-3)M to the culture lowered nitrogenase activity immediately. Other sources of fixed nitrogen had no immediate effect on nitrogenase activity, but nitrogenase synthesis decreased in the cell cycle following the one in which the fixed nitrogen was added.
通过连续分阶段培养技术使维涅兰德固氮菌OP同步化。在发酵罐内通过乙炔还原法连续测定培养物的固氮酶（氮：（受体）氧化还原酶）（EC 1.7.99.2）活性。向培养物中添加超过5×10⁻³M的NH₄⁺会立即降低固氮酶活性。其他固定氮源对固氮酶活性没有立即影响，但在添加固定氮后的细胞周期中，固氮酶合成减少。
