在测定酵母相荚膜组织胞浆菌生长活性时四氮唑盐的还原
Reduction of a tetrazolium salt in determining growth activity of yeast-phase Histoplasma capsulatum.
作者信息
Reca M E
出版信息
Appl Microbiol. 1968 Feb;16(2):236-8. doi: 10.1128/am.16.2.236-238.1968.
Abstract
A colorimetric method using a tetrazolium compound, 3,4,5-dimethylthiozalil-(1 or 2),2,5-diphenyltetrazolium bromide (TTBr), was developed for studying the growth activity of yeast-phase Histoplasma capsulatum. Materials extracted in phosphate buffer, pH 7.0, from cells at different stages of growth reduced TTBr. Colorimetric changes were correlated with enzymatic activity. Under standardized conditions specified herein, the optical density of the reduced tetrazole was an index of the growth activity of the organism.
摘要
开发了一种使用四唑化合物3,4,5-二甲基硫代唑-(1或2),2,5-二苯基溴化四唑（TTBr）的比色法，用于研究酵母相荚膜组织胞浆菌的生长活性。从处于不同生长阶段的细胞中提取的材料，用pH 7.0的磷酸盐缓冲液提取后可使TTBr还原。比色变化与酶活性相关。在本文规定的标准条件下，还原四唑的光密度是该生物体生长活性的指标。
相似文献
1
Reduction of a tetrazolium salt in determining growth activity of yeast-phase Histoplasma capsulatum.在测定酵母相荚膜组织胞浆菌生长活性时四氮唑盐的还原
Appl Microbiol. 1968 Feb;16(2):236-8. doi: 10.1128/am.16.2.236-238.1968.
2
Quantitative Microplate-Based Growth Assay for Determination of Antifungal Susceptibility of Histoplasma capsulatum Yeasts.基于微孔板的定量生长测定法用于确定荚膜组织胞浆菌酵母的抗真菌药敏性
J Clin Microbiol. 2015 Oct;53(10):3286-95. doi: 10.1128/JCM.00795-15. Epub 2015 Aug 5.
3
Adhesion of Histoplasma capsulatum to pneumocytes and biofilm formation on an abiotic surface.荚膜组织胞浆菌黏附于肺细胞和在非生物表面形成生物膜。
Biofouling. 2012;28(7):711-8. doi: 10.1080/08927014.2012.703659.
4
Colorimetric cell proliferation assay for microorganisms in microtiter plate using water-soluble tetrazolium salts.使用水溶性四氮唑盐在微量滴定板中对微生物进行比色细胞增殖测定。
J Microbiol Methods. 2008 Sep;75(1):109-16. doi: 10.1016/j.mimet.2008.05.016. Epub 2008 May 24.
5
Human lymphocyte blastogenesis induced by living and dead Histoplasma capsulatum yeasts and soluble yeast autolysate.
Sabouraudia. 1981 Jun;19(2):85-95.
6
Hydroxamic acid from Histoplasma capsulatum that displays growth factor activity.来自荚膜组织胞浆菌的显示生长因子活性的异羟肟酸。
Appl Environ Microbiol. 1981 Sep;42(3):560-3. doi: 10.1128/aem.42.3.560-563.1981.
7
Differentiation of the fungus Histoplasma capsulatum into a pathogen of phagocytes.荚膜组织胞浆菌向吞噬细胞病原体的分化。
Curr Opin Microbiol. 2017 Dec;40:1-7. doi: 10.1016/j.mib.2017.10.003. Epub 2017 Oct 27.
8
Utilization and cell-surface binding of hemin by Histoplasma capsulatum.荚膜组织胞浆菌对血红素的利用及细胞表面结合
Can J Microbiol. 2002 May;48(5):437-42. doi: 10.1139/w02-037.
9
Ferric reduction is a potential iron acquisition mechanism for Histoplasma capsulatum.铁还原是荚膜组织胞浆菌获取铁的一种潜在机制。
Infect Immun. 1999 Dec;67(12):6403-8. doi: 10.1128/IAI.67.12.6403-6408.1999.
10
Lanthionine--an organic source of sulfur for growth of yeast-phase cells of Histoplasma capsulatum.
Mycologia. 1971 Mar-Apr;63(2):432-4.
引用本文的文献
1
Varying virulence in rabbits infected with different filamentous types of Histoplasma capsulatum.感染不同丝状类型荚膜组织胞浆菌的兔子中存在不同的毒力。
J Bacteriol. 1968 Nov;96(5):1535-9. doi: 10.1128/jb.96.5.1535-1539.1968.
2
Preparation and standardization of antigens of Histoplasma capsulatum and Coccidioides immitis.荚膜组织胞浆菌和粗球孢子菌抗原的制备与标准化
Mycopathol Mycol Appl. 1970;41(1):139-53. doi: 10.1007/BF02051490.
3
The uptake and incorporation of leucine and cystine by the mycelial and yeastlike phases of Blastomyces dermatitidis.
Mycopathol Mycol Appl. 1974 Dec 18;54(4):421-33. doi: 10.1007/BF02050048.
4
Histoplasma capsulatum fails to trigger release of superoxide from macrophages.荚膜组织胞浆菌无法触发巨噬细胞释放超氧化物。
Infect Immun. 1987 Jan;55(1):29-34. doi: 10.1128/iai.55.1.29-34.1987.
5
Inhibition of murine macrophage protein kinase C activity by nonviable Histoplasma capsulatum.灭活的荚膜组织胞浆菌对小鼠巨噬细胞蛋白激酶C活性的抑制作用
Infect Immun. 1992 Jul;60(7):2683-7. doi: 10.1128/iai.60.7.2683-2687.1992.
本文引用的文献
1
The Reduction of 2,3,5-Triphenyltetrazolium Chloride by Penicillium chrysogenum.产黄青霉对氯化三苯基四氮唑的还原作用
Science. 1949 Feb 18;109(2825):169-70. doi: 10.1126/science.109.2825.169.
2
Tetrazolium as an aid to the study of tubercle bacilli.
Am J Med Sci. 1953 Apr;225(4):410-5. doi: 10.1097/00000441-195304000-00008.
3
Nature of the skin-reactive principle in culture filtrates prepared from Paracoccidioides brasiliensis.从巴西副球孢子菌制备的培养滤液中皮肤反应性原理的性质。
J Bacteriol. 1967 Jun;93(6):1741-8. doi: 10.1128/jb.93.6.1741-1748.1967.
4
Vital staining of Histoplasma capsulatum with Janus Green B.荚膜组织胞浆菌用詹纳斯绿B进行活体染色。
Sabouraudia. 1966 Jun;5(1):26-9.
Zotero 插件