Reca M E
Appl Microbiol. 1968 Feb;16(2):236-8. doi: 10.1128/am.16.2.236-238.1968.
A colorimetric method using a tetrazolium compound, 3,4,5-dimethylthiozalil-(1 or 2),2,5-diphenyltetrazolium bromide (TTBr), was developed for studying the growth activity of yeast-phase Histoplasma capsulatum. Materials extracted in phosphate buffer, pH 7.0, from cells at different stages of growth reduced TTBr. Colorimetric changes were correlated with enzymatic activity. Under standardized conditions specified herein, the optical density of the reduced tetrazole was an index of the growth activity of the organism.
开发了一种使用四唑化合物3,4,5-二甲基硫代唑-(1或2),2,5-二苯基溴化四唑（TTBr）的比色法，用于研究酵母相荚膜组织胞浆菌的生长活性。从处于不同生长阶段的细胞中提取的材料，用pH 7.0的磷酸盐缓冲液提取后可使TTBr还原。比色变化与酶活性相关。在本文规定的标准条件下，还原四唑的光密度是该生物体生长活性的指标。
