钠和钾离子在嗅觉电图产生中的作用。
The role of sodium and potassium ions in the generation of the electro-olfactogram.
作者信息
Takagi S F, Wyse G A, Kitamura H, Ito K
出版信息
J Gen Physiol. 1968 Apr;51(4):552-78. doi: 10.1085/jgp.51.4.552.
In order to clarify whether or not the electronegative olfactory mucosal potentials (EOG) are generator potentials, the effects of changed ionic enviroment were studied. The EOG decreased in amplitude and in some cases nearly or completely disappeared, when Na(+) in the bathing Ringer solution was replaced by sucrose, Li(+), choline(+), tetraethylammonium(+) (TEA), or hydrazine. In the K(+)-free Ringer solution, the negative EOG's initially increased and then decreased in amplitude. In Ringer's solution with increased K(+), the negative EOG's increased in amplitude. When K(+) was increased in exchange for Na(+) in Ringer's solution, the negative EOG's decreased, disappeared, and then reversed their polarity (Fig. 6). Next, when the K(+) was replaced by equimolar sucrose, Li(+), choline(+), TEA(+), hydrazine, or Na(+), the reversed potentials recovered completely only in Na(+)-Ringer's solution, but never in the other solutions. Thus, the essential role of Na(+) and K(+) in the negative EOG's was demonstrated. Ba(++) was found to depress selectively the electropositive EOG, but it hardly decreased and never increased the negative EOG. Hence, it is concluded that Ba(++) interferes only with Cl(-) influx, and that the negative EOG's are elicited by an increase in permeability of the olfactory receptive membrane to Na(+) and K(+), but not to Cl(-). From the ionic mechanism it is inferred that the negative EOG's are in most cases composites of generator and positive potentials.
为了阐明负性嗅觉黏膜电位（EOG）是否为发生器电位，研究了离子环境变化的影响。当浴槽中的任氏液中的Na⁺被蔗糖、Li⁺、胆碱⁺、四乙铵⁺（TEA）或肼替代时，EOG的幅度减小，在某些情况下几乎完全消失。在无K⁺的任氏液中，负性EOG最初幅度增加，然后减小。在K⁺浓度增加的任氏液中，负性EOG的幅度增加。当在任氏液中增加K⁺以交换Na⁺时，负性EOG减小、消失，然后极性反转（图6）。接下来，当K⁺被等摩尔的蔗糖、Li⁺、胆碱⁺、TEA⁺、肼或Na⁺替代时，反转电位仅在Na⁺ - 任氏液中完全恢复，而在其他溶液中从未恢复。因此，证明了Na⁺和K⁺在负性EOG中的重要作用。发现Ba²⁺选择性地抑制正性EOG，但几乎不降低且从不增加负性EOG。因此，得出结论，Ba²⁺仅干扰Cl⁻内流，负性EOG是由嗅觉感受膜对Na⁺和K⁺而非Cl⁻的通透性增加引起的。从离子机制推断，负性EOG在大多数情况下是发生器电位和正性电位的复合物。
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