Britton S, Celada F
Immunology. 1968 Apr;14(4):503-9.
The disappearance of immunogenicity of human serum albumin (HSA) was measured by injection of 1 μg of this protein in normal mice and by transferring to them, at intervals, a fixed number of HSA-sensitized syngeneic spleen cells: subsequently the antigen binding capacity of the serum was determined. This system served to reveal the residual amount of immunogen present in the animal, since under these conditions the adoptive secondary response is proportional to the HSA available, and no antibody is formed by the host. The half-life of HSA immunogenicity was 17½ hours. A similar rate of decline ( = 16½ hours) was found by following I coupled to the HSA in the circulation of the same mice. This demonstrates that the albumin antigen is not stored in an active form in non-pre-immunized mice.
通过向正常小鼠注射1μg这种蛋白质，并每隔一段时间向它们转移固定数量的人血清白蛋白（HSA）致敏的同基因脾细胞，来测定人血清白蛋白（HSA）免疫原性的消失情况：随后测定血清的抗原结合能力。该系统用于揭示动物体内存在的免疫原残留量，因为在这些条件下，过继性二次反应与可用的HSA成正比，且宿主不形成抗体。HSA免疫原性的半衰期为17.5小时。通过追踪与同一小鼠循环中的HSA偶联的碘，发现了类似的下降速率（=16.5小时）。这表明白蛋白抗原在未预先免疫的小鼠中不是以活性形式储存的。
