McConahey P J, Cerottini J C, Dixon F J
J Exp Med. 1968 May 1;127(5):1003-11. doi: 10.1084/jem.127.5.1003.
Using passively administered isotope-labeled anti-KLH to suppress the antibody response of rabbits to KLH, we have attempted to estimate the amount of antigen actually involved in stimulating antibody formation. Single and paired label tracer studies of passively administered anti-KLH IgG indicated that from 0.7 to 2.9 microg were utilized or involved by the antigen in the course of a 90% suppression of the response to 2 mg KLH. Tracer studies of labeled anti-KLH F(ab')(2) fragments revealed the retention of from 2 to 3 microg of these fragments in the entire rabbit during a 60% suppression of the response to 1 mg KLH. Based on previously determined ratios of mixtures of KLH and suppressive amounts of anti-KLH in adjuvant, the antibody utilization data were converted to the probable amount of antigen or immunogen involved. It appears that after an injection of 2 mg of KLH approximately 0.2-0.5 microg of antigen persisted and reacted with antibody given 24 hr later. Since all of this persisting, reactive antigen may not be immunogenic, the above estimate of immunogen is probably high, but may serve to establish upper limits for the amounts of immunogen involved in stimulating antibody formation and provide a meaningful frame of reference for antigen tracer studies.
我们通过被动给予同位素标记的抗钥孔戚血蓝蛋白（KLH）来抑制家兔对KLH的抗体反应，试图估算实际参与刺激抗体形成的抗原量。对被动给予的抗KLH IgG进行单标记和双标记示踪研究表明，在对2mg KLH的反应被抑制90%的过程中，抗原利用或涉及了0.7至2.9微克。对标记的抗KLH F(ab')(2)片段的示踪研究显示，在对1mg KLH的反应被抑制60%的过程中，整个家兔体内保留了2至3微克的这些片段。根据先前确定的KLH与佐剂中抑制量的抗KLH混合物的比例，将抗体利用数据换算为可能涉及的抗原或免疫原的量。注射2mg KLH后，似乎约有0.2 - 0.5微克的抗原持续存在，并与24小时后给予的抗体发生反应。由于所有这些持续存在的反应性抗原可能并非都具有免疫原性，上述免疫原的估计值可能偏高，但或许有助于确定刺激抗体形成所涉及的免疫原量的上限，并为抗原示踪研究提供一个有意义的参考框架。
