Zänker K S, Schiebel W
Biochem J. 1978 May 1;171(2):445-51. doi: 10.1042/bj1710445.
Cytoplasmic DNA polymerase (DNA deoxynucleotidyltransferase, EC 2.7.7.7) was partially purified from Physarum polycephalum. The first step of the purification procedure utilized the fact that the enzyme on gel filtration behaves in anomalous fashion. The second step was either ion-exchange chromatography or sucrose-density-gradient centrifugation. The partially purified DNA polymerase was heterogeneous and at least four species with different sedimentation coefficients (5.5S, 7.2S, 8.6S and 11.5S) were detected. Calculated molecular weights indicated a tendency for stoicheiometric polypeptide aggregation, accompanied by an alteration of the three-dimensional structure froma compact spheroid to a more open elliptical form. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and computed molecular weights suggest an active protomer in the range of 113000 daltons; all data pertain to I 0.045, which was maintained during the whole procedure.
从多头绒泡菌中部分纯化出细胞质DNA聚合酶（DNA脱氧核苷酸转移酶，EC 2.7.7.7）。纯化过程的第一步利用了该酶在凝胶过滤中表现出异常行为这一事实。第二步是离子交换色谱法或蔗糖密度梯度离心法。部分纯化的DNA聚合酶具有异质性，检测到至少四种具有不同沉降系数（5.5S、7.2S、8.6S和11.5S）的种类。计算得到的分子量表明存在化学计量多肽聚集的趋势，同时伴随着三维结构从紧密球体变为更开放的椭圆形的变化。十二烷基硫酸钠/聚丙烯酰胺凝胶电泳和计算得到的分子量表明活性单体在113000道尔顿范围内；所有数据均与I 0.045有关，在整个过程中保持不变。
