在胰蛋白酶催化特定环活化酰苯胺水解过程中四面体中间体的检测。
Detection of a tetrahedral intermediate in the trypsin-catalysed hydrolysis of specific ring-activated anilides.
作者信息
Petkov D D
出版信息
Biochim Biophys Acta. 1978 Apr 12;523(2):538-41. doi: 10.1016/0005-2744(78)90057-8.
PMID:566121
Abstract
The substituent dependence for kcat/Km of trypsin anilide hydrolysis is consistent with a rate-limiting general acid-base catalysed breakdown of a tetrahedral intermediate. The formation and disappearance of this intermediate during the hydrolysis of alpha-N-acetyl-L-lysin p-nitroanilide is observed in stopped-flow experiments.
摘要
胰蛋白酶催化苯胺水解反应的催化常数与米氏常数之比（kcat/Km）对取代基的依赖性，与四面体中间体通过限速的一般酸碱催化分解的情况相符。在α-N-乙酰-L-赖氨酸对硝基苯胺水解过程中该中间体的形成和消失，在停流实验中得以观察。
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