• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Current problems in mechanistic studies of serine and cysteine proteinases.

作者信息

Polgár L, Halász P

出版信息

Biochem J. 1982 Oct 1;207(1):1-10. doi: 10.1042/bj2070001.

DOI:10.1042/bj2070001
PMID:6758764
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1153816/
Abstract
摘要

相似文献

1
Current problems in mechanistic studies of serine and cysteine proteinases.丝氨酸蛋白酶和半胱氨酸蛋白酶机制研究中的当前问题。
Biochem J. 1982 Oct 1;207(1):1-10. doi: 10.1042/bj2070001.
2
The basic difference in catalyses by serine and cysteine proteinases resides in charge stabilization in the transition state.
J Theor Biol. 1986 Aug 7;121(3):323-6. doi: 10.1016/s0022-5193(86)80111-4.
3
Mechanism of the reaction of papain with substrate-derived diazomethyl ketones. Implications for the difference in site specificity of halomethyl ketones for serine proteinases and cysteine proteinases and for stereoelectronic requirements in the papain catalytic mechanism.木瓜蛋白酶与底物衍生的重氮甲基酮的反应机制。对卤甲基酮对丝氨酸蛋白酶和半胱氨酸蛋白酶的位点特异性差异以及木瓜蛋白酶催化机制中的立体电子要求的影响。
Biochem J. 1978 Nov 1;175(2):761-4. doi: 10.1042/bj1750761.
4
Evidence that the carboxy groups of Asp158 in papain and caricain have abnormally low pKa values and thus do not contribute the key ionisations with pKa 4 that generate catalytic competence.
Biochem Soc Trans. 1997 Feb;25(1):90S. doi: 10.1042/bst025090s.
5
Mechanism of action of cysteine proteinases: oxyanion binding site is not essential in the hydrolysis of specific substrates.半胱氨酸蛋白酶的作用机制：氧阴离子结合位点在特定底物的水解中并非必不可少。
Biochemistry. 1985 Jan 29;24(3):606-9. doi: 10.1021/bi00324a010.
6
Rapid kinetic studies and structural determination of a cysteine proteinase mutant imply that residue 158 in caricain has a major effect upon the ability of the active site histidine to protonate a dipyridyl probe.半胱氨酸蛋白酶突变体的快速动力学研究和结构测定表明，木瓜蛋白酶中的158位残基对活性位点组氨酸使二吡啶探针质子化的能力有重大影响。
Biochemistry. 1996 Nov 26;35(47):14763-72. doi: 10.1021/bi960949r.
7
ECEPE proteins: a novel family of eukaryotic cysteine proteinases.ECEPE蛋白：一类新型真核生物半胱氨酸蛋白酶。
Trends Biochem Sci. 2004 Oct;29(10):524-6. doi: 10.1016/j.tibs.2004.08.003.
8
A marked gradation in active-centre properties in the cysteine proteinases revealed by neutral and anionic reactivity probes. Reactivity characteristics of the thiol groups of actinidin, ficin, papain and papaya peptidase A towards 4,4'-dipyridyl disulphide and 5,5'-dithiobis-(2-nitrobenzoate) dianion.通过中性和阴离子反应性探针揭示的半胱氨酸蛋白酶活性中心性质的显著分级。肌动蛋白水解酶、无花果蛋白酶、木瓜蛋白酶和木瓜蛋白酶A的巯基对4,4'-二吡啶二硫化物和5,5'-二硫代双-(2-硝基苯甲酸)二阴离子的反应特性。
Biochem J. 1983 Mar 1;209(3):873-9. doi: 10.1042/bj2090873.
9
Ionization behavior of the histidine residue in the catalytic triad of serine proteases. Mechanistic implications.
J Biol Chem. 1973 Dec 10;248(23):8306-8.
10
Insight into catalytic mechanism of papain-like cysteine proteinases: the case of D158.木瓜蛋白酶样半胱氨酸蛋白酶催化机制的深入研究：以D158为例。
Appl Biochem Biotechnol. 2004 Jul-Sep;118(1-3):171-5. doi: 10.1385/abab:118:1-3:171.

引用本文的文献

1
Molecular basis of specificity and deamidation of eIF4A by Burkholderia Lethal Factor 1.eIF4A 被伯克霍尔德菌致死因子 1 特异性脱酰胺的分子基础。
Commun Biol. 2022 Mar 28;5(1):272. doi: 10.1038/s42003-022-03186-2.
2
Catalytic Site p Values of Aspartic, Cysteine, and Serine Proteases: Constant pH MD Simulations.天冬氨酸、半胱氨酸和丝氨酸蛋白酶的催化位点 p 值：恒 pHMD 模拟。
J Chem Inf Model. 2020 Jun 22;60(6):3030-3042. doi: 10.1021/acs.jcim.0c00190. Epub 2020 May 29.
3
Distinct Roles of Catalytic Cysteine and Histidine in the Protease and Ligase Mechanisms of Human Legumain As Revealed by DFT-Based QM/MM Simulations.基于密度泛函理论的量子力学/分子力学模拟揭示催化半胱氨酸和组氨酸在人豆球蛋白蛋白酶和连接酶机制中的不同作用
ACS Catal. 2017 Sep 1;7(9):5585-5593. doi: 10.1021/acscatal.7b01505. Epub 2017 Jul 14.
4
Role of proteases in the pathophysiology of cardiac disease.蛋白酶在心脏疾病病理生理学中的作用。
Mol Cell Biochem. 2004 Aug;263(1):241-56. doi: 10.1023/B:MCBI.0000041865.63445.40.
5
An Unusual Member of the Papain Superfamily: Mapping the Catalytic Cleft of the Marasmius oreades agglutinin (MOA) with a Caspase Inhibitor.木瓜蛋白酶超家族的一个特殊成员：用半胱天冬酶抑制剂绘制小皮伞凝集素（MOA）的催化裂隙
PLoS One. 2016 Feb 22;11(2):e0149407. doi: 10.1371/journal.pone.0149407. eCollection 2016.
6
Specific Inter-residue Interactions as Determinants of Human Monoacylglycerol Lipase Catalytic Competency: A ROLE FOR GLOBAL CONFORMATIONAL CHANGES.特定残基间相互作用作为人单酰甘油脂肪酶催化活性的决定因素：全局构象变化的作用
J Biol Chem. 2016 Feb 5;291(6):2556-65. doi: 10.1074/jbc.M115.670257. Epub 2015 Nov 10.
7
Trichomonas vaginalis Cysteine Proteinases: Iron Response in Gene Expression and Proteolytic Activity.阴道毛滴虫半胱氨酸蛋白酶：基因表达和蛋白水解活性中的铁反应
Biomed Res Int. 2015;2015:946787. doi: 10.1155/2015/946787. Epub 2015 May 18.
8
Theoretical insight into the mechanism for the inhibition of the cysteine protease cathepsin B by 1,2,4-thiadiazole derivatives.1,2,4-噻二唑衍生物抑制半胱氨酸蛋白酶组织蛋白酶B机制的理论洞察
J Mol Model. 2014 Jun;20(6):2254. doi: 10.1007/s00894-014-2254-0. Epub 2014 Jun 1.
9
Design, synthesis, evaluation and thermodynamics of 1-substituted pyridylimidazo[1,5-a]pyridine derivatives as cysteine protease inhibitors.1-取代吡啶基咪唑并[1,5-a]吡啶衍生物作为半胱氨酸蛋白酶抑制剂的设计、合成、评价及热力学研究。
PLoS One. 2013 Aug 5;8(8):e69982. doi: 10.1371/journal.pone.0069982. Print 2013.
10
Reaction pathway and free energy profile for papain-catalyzed hydrolysis of N-acetyl-Phe-Gly 4-nitroanilide.木瓜蛋白酶催化 N-乙酰-Phe-Gly 4-硝基苯胺水解的反应途径和自由能图。
Biochemistry. 2013 Jul 30;52(30):5145-54. doi: 10.1021/bi400629r. Epub 2013 Jul 17.

本文引用的文献

1
DIRECT EVIDENCE FOR AN ACYLATED THIOL AS AN INTERMEDIATE IN PAPAIN- AND FICIN-CATALYSED HYDROLYSES.酰化硫醇作为木瓜蛋白酶和无花果蛋白酶催化水解中间体的直接证据。
Biochem J. 1965 Jul;96(1):189-93. doi: 10.1042/bj0960189.
2
ACTIVATION OF STREPTOCOCCAL PROTEINASE AND ITS ZYMOGEN BY BACTERIAL CELL WALLS.细菌细胞壁对链球菌蛋白酶及其酶原的激活作用。
Nature. 1965 Apr 3;206:33-4. doi: 10.1038/206033a0.
3
MECHANISM OF ACTION OF PROTEOLYTIC ENZYMES.蛋白水解酶的作用机制
Annu Rev Biochem. 1965;34:49-76. doi: 10.1146/annurev.bi.34.070165.000405.
4
Analogy between active sites of cathepsin B(1) and papain.组织蛋白酶B(1)与木瓜蛋白酶活性位点之间的类比。
FEBS Lett. 1970 Dec 11;11(4):287-288. doi: 10.1016/0014-5793(70)80550-6.
5
Perturbations in the free energy and enthalpy of ionization of histidine-159 at the active site of papain as determined by fluorescence spectroscopy.通过荧光光谱法测定木瓜蛋白酶活性位点处组氨酸-159的电离自由能和焓的扰动。
Biochemistry. 1981 Jan 6;20(1):52-8. doi: 10.1021/bi00504a600.
6
Effect of cysteine-25 on the ionization of histidine-159 in papain as determined by proton nuclear magnetic resonance spectroscopy. Evidence for a his-159--Cys-25 ion pair and its possible role in catalysis.通过质子核磁共振光谱法测定半胱氨酸-25对木瓜蛋白酶中组氨酸-159电离的影响。组氨酸-159与半胱氨酸-25离子对的证据及其在催化中的可能作用。
Biochemistry. 1981 Jan 6;20(1):48-51. doi: 10.1021/bi00504a009.
7
Soybean trypsin inhibitor (Kunitz) and its complex with trypsin. Carbon-13 nuclear magnetic resonance studies of the reactive site arginine.
Biochemistry. 1980 Dec 9;19(25):5703. doi: 10.1021/bi00566a006.
8
Neutron diffraction identifies His 57 as the catalytic base in trypsin.
Nature. 1980 Nov 27;288(5789):414-6. doi: 10.1038/288414a0.
9
Is the thiolate--imidazolium ion pair the catalytically important form of papain?硫醇盐 - 咪唑鎓离子对是木瓜蛋白酶具有催化活性的重要形式吗？
FEBS Lett. 1980 Feb 11;110(2):319-22. doi: 10.1016/0014-5793(80)80101-3.
10
Solvent isotope effects on tautomerization equilibria of papain and model thiolamines.溶剂同位素对木瓜蛋白酶和模型硫醇胺互变异构平衡的影响。
FEBS Lett. 1980 Feb 11;110(2):313-8. doi: 10.1016/0014-5793(80)80100-1.