用于凝血酶凝血活性测定的显色底物的不可靠性。
Unreliability of chromogenic substrates for assay of the clotting activity of thrombin.
作者信息
Gaffney P J, Miller-Andersson M, Kirkwood T B
出版信息
Haemostasis. 1978;7(2-3):109-12. doi: 10.1159/000214246.
PMID:566247
Abstract
The rapid loss in clotting activity of commercially available thrombins during storage is not reflected by their activity as measured by synthetic chromogenic substrates. It is suggested that conformational changes occurring during storage can affect the binding sites of thrombin for fibrinogen, whereas small synthetic substrates are incapable of monitoring these changes.
摘要
市售凝血酶在储存期间凝血活性的快速丧失，并未通过合成生色底物所测得的活性表现出来。这表明储存期间发生的构象变化会影响凝血酶与纤维蛋白原的结合位点，而小型合成底物无法监测这些变化。
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