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Radioautographic localization of deoxythymidine triphosphate in Tradescantia pollen grains during DNA synthesis.DNA合成期间紫露草花粉粒中三磷酸脱氧胸苷的放射自显影定位
J Cell Biol. 1968 Sep;38(3):509-14. doi: 10.1083/jcb.38.3.509.
2
Effect of 2'-deoxyadenosine on thymidine utilization by tradescantia pollen grains.2'-脱氧腺苷对紫露草花粉粒胸苷利用的影响。
Plant Physiol. 1967 Jul;42(7):907-10. doi: 10.1104/pp.42.7.907.
3
DNA polymerase and DNA nuclease activities in S-competent and S-incompetent nuclei from Tradescantia pollen grains.
Exp Cell Res. 1971 Nov;69(1):25-8. doi: 10.1016/0014-4827(71)90305-3.
4
[The development of pollen grains and formation of pollen tubes in higher plants : V. The division of the generative nucleus in the pollen tube of Tradescantia paludosa].[高等植物花粉粒的发育及花粉管的形成：V. 紫露草花粉管中生殖核的分裂]
Theor Appl Genet. 1973 Jan;43(6):269-75. doi: 10.1007/BF00277787.
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Labelling nuclei of progressively developing pollen mother cells and pollen with tritiated thymidine in Tradescantia.用氚标记胸腺嘧啶核苷标记紫露草中逐渐发育的花粉母细胞和花粉的细胞核。
Stain Technol. 1970 May;45(3):105-8. doi: 10.3109/10520297009085349.
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A radioautographic study with H3-thymidine on adrenal medulla nuclei of rats intermittently exposed to cold.用H3-胸腺嘧啶核苷对间歇性暴露于寒冷环境的大鼠肾上腺髓质细胞核进行放射自显影研究。
J Cell Biol. 1966 Jan;28(1):9-19. doi: 10.1083/jcb.28.1.9.
7
Isolation and separation of S-competent and S-incompetent nuclei from Tradescantia pollen grains.
Exp Cell Res. 1971 Nov;69(1):29-32. doi: 10.1016/0014-4827(71)90306-5.
8
[The development of pollen grains and formation of pollen tubes in higher plants : I. Quantitative measurements of the DNA-content of generative and vegetative nuclei in the pollen grain and pollen tube of Petunia hybrida mutants].[高等植物花粉粒的发育及花粉管的形成：I. 矮牵牛突变体花粉粒和花粉管中生殖核与营养核DNA含量的定量测定]
Theor Appl Genet. 1971 Jan;41(8):338-51. doi: 10.1007/BF00277334.
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Intracellular amounts of nucleic acids and protein during pollen grain growth in Tradescantia.紫露草花粉粒生长过程中细胞内核酸和蛋白质的含量
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10
Analysis of tritium incorporation into individual cells by autoradiography of squash preparations.通过压片标本放射自显影分析氚掺入单个细胞的情况。
J Biophys Biochem Cytol. 1960 Oct;8(2):327-31. doi: 10.1083/jcb.8.2.327.

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Nuclear DNA replicates during zygote development in Arabidopsis and Torenia fournieri.在拟南芥和四翅滨藜的合子发育过程中，核 DNA 会进行复制。
Plant Physiol. 2021 Feb 25;185(1):137-145. doi: 10.1093/plphys/kiaa014.

本文引用的文献

1
Effect of 2'-deoxyadenosine on thymidine utilization by tradescantia pollen grains.2'-脱氧腺苷对紫露草花粉粒胸苷利用的影响。
Plant Physiol. 1967 Jul;42(7):907-10. doi: 10.1104/pp.42.7.907.
2
A comparison between thin-layer chromatography and paper chromatography of nucleic acid derivatives.核酸衍生物的薄层色谱法与纸色谱法的比较。
Biochem Biophys Res Commun. 1962 Jan 24;6:452-7. doi: 10.1016/0006-291x(62)90374-1.
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Giemsa staining of autoradiograms prepared with stripping film.用剥离膜制备的放射自显影片的吉姆萨染色。
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STUDIES OF THE SITE OF ACTION OF INSULIN IN THE PENETRATION OF MUSCLE FIBERS BY 14C-D-XYLOSE BY MEANS OF A GENERAL AUTORADIOGRAPHIC METHOD FOR LOCALIZATION OF DIFFUSIBLE SUBSTANCES.利用一种用于定位可扩散物质的通用放射自显影方法，研究胰岛素在¹⁴C-D-木糖穿透肌纤维过程中的作用位点。
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High-resolution autoradiography. I. Methods.高分辨率放射自显影术。I. 方法。
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6
Differential uptake of 3Hthymidine into the soluble fraction of single bone marrow cells, determined by autoradiography.通过放射自显影法测定3H胸腺嘧啶核苷在单个骨髓细胞可溶部分中的差异摄取。
Exp Cell Res. 1962 Sep;27:474-84. doi: 10.1016/0014-4827(62)90013-7.
7
Uptake of thymidine and synthesis of deoxyribonucleic acid in mouse ascites cells.小鼠腹水细胞中胸苷的摄取及脱氧核糖核酸的合成
Nature. 1960 Aug 13;187:614-5. doi: 10.1038/187614a0.
8
A method for the separation of nucleotides by concave gradient elution.一种通过凹形梯度洗脱分离核苷酸的方法。
Biochim Biophys Acta. 1958 Mar;27(3):618-24. doi: 10.1016/0006-3002(58)90395-0.
9
Some reactions of adenosine and inosine phosphates in animal tissues.动物组织中腺苷酸和肌苷酸的一些反应。
Biochim Biophys Acta. 1953 Sep-Oct;12(1-2):172-80. doi: 10.1016/0006-3002(53)90136-x.

DNA合成期间紫露草花粉粒中三磷酸脱氧胸苷的放射自显影定位

Radioautographic localization of deoxythymidine triphosphate in Tradescantia pollen grains during DNA synthesis.

作者信息

Takats S T

出版信息

J Cell Biol. 1968 Sep;38(3):509-14. doi: 10.1083/jcb.38.3.509.

DOI:10.1083/jcb.38.3.509
PMID:5664221
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2108366/
Abstract

Tradescantia pollen grains, isolated during the period of DNA synthesis in the generative cell, accumulate deoxythymidine triphosphate (dTTP)-(3)H after incubation with thymidine-(3)H in the presence of millimolar deoxyadenosine. Most of this dTTP-(3)H was found to resist extraction by the fixative, cold ethanol-acetic acid, and its location was investigated by radioautography with thin, dry emulsion. Substantial binding of dTTP-(3)H occurred as an artifact; but when nuclei were isolated from the fixed pollen grains by sonication, it was found that they were differentially labeled: generative nuclei contained dTTP-(3)H, vegetative nuclei did not. This observation is discussed and is interpreted as evidence supporting the idea that thymidine is phosphorylated only in the generative cell of the pollen grain.

摘要

在生殖细胞进行DNA合成期间分离得到的紫露草花粉粒，在含有毫摩尔脱氧腺苷的情况下与胸腺嘧啶核苷 -（3）H一起孵育后，积累了三磷酸脱氧胸苷（dTTP） -（3）H。发现大部分这种dTTP -（3）H能抵抗固定剂冷乙醇 - 乙酸的提取，并用薄的干乳胶放射自显影法研究其定位。dTTP -（3）H的大量结合是一种假象；但是当通过超声处理从固定的花粉粒中分离出细胞核时，发现它们有差异标记：生殖细胞核含有dTTP -（3）H，营养细胞核则没有。对这一观察结果进行了讨论，并解释为支持胸腺嘧啶仅在花粉粒的生殖细胞中磷酸化这一观点的证据。