Department of Biology, Temple University, Philadelphia, Pennsylvania 19122.
Plant Physiol. 1967 Jul;42(7):907-10. doi: 10.1104/pp.42.7.907.
The uptake of (3)H-thymidine into pollen grains of Tradescantia paludosa was studied in the presence of 2'-deoxyadenosine. 1) Millimolar deoxyadenosine caused an immediate inhibition of incorporation of (3)H-thymidine into DNA extracted with hot trichloroacetic acid. 2) The radioactivity in acid-soluble derivatives of (3)H-thymidine was examined by paper chromatography and, following incubation of pollen grains in the presence of millimolar deoxyadenosine, was found to be increased several-fold in (3)H-deoxythymidine triphosphate. 3) The time-course of inhibition showed that the acid-soluble derivatives of (3)H-thymidine accumulated initially at a rate unaffected by deoxyadenosine, despite the nearly complete inhibition of incorporation of (3)H-thymidine into DNA. This is discussed in relation to possible mechanisms of inhibition by deoxyadenosine.
在存在 2′-脱氧腺苷的情况下，研究了 Tradescantia paludosa 花粉粒中 (3)H-胸腺嘧啶核苷的摄取。1）毫摩尔脱氧腺苷立即抑制用热三氯乙酸提取的 DNA 中 (3)H-胸腺嘧啶核苷的掺入。2）通过纸层析法检查 (3)H-胸腺嘧啶核苷的酸溶性衍生物，并且在花粉粒在毫摩尔脱氧腺苷存在下孵育后，发现 (3)H-脱氧胸苷三磷酸的放射性增加了几倍。3）抑制的时程表明，尽管 (3)H-胸腺嘧啶核苷掺入 DNA 的几乎完全抑制，但酸溶性 (3)H-胸腺嘧啶核苷衍生物最初以不受脱氧腺苷影响的速率积累。这与脱氧腺苷抑制的可能机制有关。
