Matsuura S, Morimoto T, Nagata S, Tashiro Y
J Cell Biol. 1968 Sep;38(3):589-603. doi: 10.1083/jcb.38.3.589.
Cytolytic processes in posterior silk gland cells of the silkworm, Bombyx mori, during metamorphosis from larva to pupa have been studied. During this stage, the wet weight and the amounts of RNA and protein of the gland decrease rapidly and markedly, while the amount of DNA decreases slowly and slightly. The ultrastructural changes observed at the beginning of the prepupal stage consist of the appearance or the increase in the number of autophagosomes containing endoplasmic reticulum (ER), or "early autophagosomes" as we have called them, which seem to be gradually transformed to autolysosomes. A number of usual lysosomes, which frequently contain myelin figures, also appear in the cytoplasm. Sometimes they fuse with each other to form large conglomerates. In the middle of the prepupal stage, a number of smooth membrane-bounded vacuoles appear in cytoplasm. Towards the end of the prepupal stage the partition or sequestration of cytoplasm was observed. Thus large autophagosomes containing cytoplasmic organelles such as rough ER and/or mitochondria are formed. The nucleus is partitioned in a similar way by smooth membranes, and then autophagosomes containing condensed chromatin blocks are formed. These various kinds of autophagosomes, or "late autophagosomes" as we have generally called them, are continuously released into the hemolymph until the gland is completely disintegrated.
对家蚕（Bombyx mori）从幼虫到蛹变态过程中后部丝腺细胞的溶细胞过程进行了研究。在此阶段，丝腺的湿重、RNA和蛋白质含量迅速且显著下降，而DNA含量下降缓慢且轻微。在化蛹前期开始时观察到的超微结构变化包括含有内质网（ER）的自噬体（我们称之为“早期自噬体”）的出现或数量增加，这些自噬体似乎逐渐转变为自溶酶体。许多通常含有髓鞘样结构的溶酶体也出现在细胞质中。有时它们相互融合形成大的聚集体。在化蛹前期中期，细胞质中出现许多光滑膜包被的液泡。在化蛹前期接近尾声时，观察到细胞质的分隔或隔离。这样就形成了含有粗面内质网和/或线粒体等细胞质细胞器的大型自噬体。细胞核也以类似的方式被光滑膜分隔，然后形成含有凝聚染色质块的自噬体。这些各种各样的自噬体，即我们通常所说的“晚期自噬体”，不断释放到血淋巴中，直到丝腺完全解体。
