两性霉素B对蟾蜍膀胱的作用：沿两条途径进行钠转运的证据。

Action of amphotericin B on the toad bladder: evidence for sodium transport along two pathways.

作者信息

Bentley P J

出版信息

J Physiol. 1968 Jun;196(3):703-11. doi: 10.1113/jphysiol.1968.sp008531.

DOI:10.1113/jphysiol.1968.sp008531

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1351772/

Abstract

The relationship of short-circuit current (SCC, sodium transport) and potential difference (p.d.) across the toad bladder to the concentration of sodium on the mucosal side was measured. Maximal values were attained when the Na concentration was 10 mM for SCC and 30 mM for p.d.2. Amphotericin B increased SCC across bladders bathed on their mucosal surface with isotonic (115 mM) NaCl solution and this effect was not inhibited by cyanide and iodoacetate. The osmotic permeability of the bladder was also increased by amphotericin B.3. Vasopressin and aldosterone increased sodium transport from solutions of low Na concentration, but amphotericin B was ineffective until a level of about 40-60 mM was present.4. The evidence suggests two pathways for sodium transport across the bladder distinguished by differences in their electrochemical gradients with the fluid at the mucosal side.

摘要

测量了蟾蜍膀胱的短路电流（SCC，即钠转运）和跨膜电位差（p.d.）与黏膜侧钠浓度的关系。当钠浓度为10 mM时，SCC达到最大值；当钠浓度为30 mM时，p.d.达到最大值。
两性霉素B增加了用等渗（115 mM）NaCl溶液浸泡其黏膜表面的膀胱的SCC，且这种作用不受氰化物和碘乙酸盐的抑制。两性霉素B还增加了膀胱的渗透通透性。
血管加压素和醛固酮增加了低钠浓度溶液中的钠转运，但在钠浓度约为40 - 60 mM之前，两性霉素B无效。
证据表明，钠通过膀胱转运有两条途径，这两条途径的区别在于它们与黏膜侧液体的电化学梯度不同。