CO2-dependent component of the neurogenic vascular tone in the cat.

Complete vascular isolation of the hindlimbs was performed in vagotomized cats under Sodium Pentobarbital anesthesia. The hindlimbs were perfused at constant flow with blood kept at a constant and physiological PO2, PCO2, and pH values by means of a specially designed pump-oxygenator system. The animals were hyperventilated with different CO2 mixtures (0%, 5%, 7% and 10% in O2) thereby changing blood gases and pH levels in the upper body but not in the hindlimb vascular bed. At body PaCO2 (mm Hg) of 13.7 +/- 1.0 (means +/- SE), 30.6 +/- 1.05, 40,4 +/- 0,9 and 58.4 +/- 2.9 the hindlimb perfusion pressure (mm Hg) was, respectively 124 +/- 7.6, 138 +/- 7.4, 156 +/- 11.9 and 187 +/- 15.1. These changes in perfusion pressure were still present after complete peripheral chemoreceptor denervation but were abolished after section of the spinal cord at the T5 level. Since hindlimb perfusion pressure fell when body PaCO2 was lowered below physiological levels it is concluded that part of the neurogenic vascular tone of the hindlimbs is maintained by a CO2 mediated stimulation of supraspinal structures.