[Histochemical behavior of acetylcholinesterase in the diaphragm and that of unspecific esterase in the hypothalamus, testes and liver of rats in relation to organophosphates].

The enzyme behaviour of acetylcholinesterase in diaphragm and that of unspecific esterase in liver, testes and hypothalamus were investigated in view of various organophosphates on total-preparation and cryostat section of 10 male wistar rats. It had pointed out that the blockade was dependent upon the concentration as well as the acid possessing P = O-binding proved to be appropriate for in vitro experiment. The most intense blockade of unspecific esterase was reached in tanycytependym of the third ventricle. The acetylcholinesterase in the motor endplate of diaphragm was less influenced. From these findings follows that a direct conclusion for in vivo toxicity can not be drawn from in vitro results. The significance of the chemical structure of the substance utilized was discussed concerning its inhibitory effect.