Kovatchev S, Eibl H
Adv Exp Med Biol. 1978;101:221-6. doi: 10.1007/978-1-4615-9071-2_21.
The transfer of the phosphatidyl residue from egg phosphatidylcholine to primary alkanols catalyzed by phospholipase D was systematically investigated. It was demonstrated that 1) the chain length of the alkanols is of critical importance, e.g. transphosphatidylation does not occur to alkanols or alkandiols with more than six carbon atoms; 2) double or triple bonds in the acceptor molecule do not influence the transfer reaction; 3) fluorine is tolerated in the acceptor molecule, but the transfer rate decreases with increasing atomic weight from chlorine to iodine. Synthetic phosphatidylcholines with large variations in the apolar part of the molecule, the phosphorylcholines of 1.2-diacyl-sn-glycerol, Acyl-propandiol-(1.3) and 1.2-cyclopentadecylmethylidene-glycerol, have been successfully used in the transfer reaction. Transesterification is an attractive route for the synthesis of phospholipids differing in the polar part of the molecule.
对磷脂酶D催化的从鸡蛋磷脂酰胆碱到伯链烷醇的磷脂酰残基转移进行了系统研究。结果表明：1）链烷醇的链长至关重要，例如，对于碳原子数超过6个的链烷醇或链烷二醇不会发生转磷脂酰基反应；2）受体分子中的双键或三键不影响转移反应；3）受体分子中可耐受氟，但随着原子量从氯增加到碘，转移速率降低。分子非极性部分有很大差异的合成磷脂酰胆碱、1,2 -二酰基 - sn -甘油的磷酰胆碱、酰基 - 丙二醇 - (1,3)和1,2 -环十五亚甲基甘油已成功用于转移反应。酯交换是合成分子极性部分不同的磷脂的一条有吸引力的途径。
