大鼠肝脏中脂蛋白脂肪酶的功能状态。
The functional status of lipoprotein lipase in rat liver.
作者信息
Mayes P A, Felts J M
出版信息
Biochem J. 1968 Jul;108(3):483-7. doi: 10.1042/bj1080483.
Abstract
- Acetone-dried powders of liver and heart tissues from rats given a high-carbohydrate diet or a fat meal were assayed for lipoprotein lipase activity. Heart tissue showed typical lipoprotein lipase activity, whereas none was detected in liver by the usual assay procedures. 2. When mixed acetone-dried powders were prepared from heart plus liver, there was a marked suppression of the expected activity, indicating that an inhibitor was present in the liver. This inhibition was partially overcome in the presence of relatively large amounts of heparin. 3. Lipoprotein lipase was also detected in liver alone when large quantities of heparin were added to the assay system. 4. No increase in lipoprotein lipase activity in either liver or heart was detected when rats were given a fat meal. 5. It is concluded that the liver of the rat contains lipoprotein lipase that is normally present in an inactive state. The results imply that a heparinase is the agent responsible for the inactivation. 6. The significance of the non-functional status of lipoprotein lipase in the liver is discussed. The results support the view that direct hydrolysis of plasma triglycerides by the liver is not a significant physiological process.
摘要
- 对给予高碳水化合物饮食或脂肪餐的大鼠的肝脏和心脏组织的丙酮干粉进行脂蛋白脂肪酶活性测定。心脏组织显示出典型的脂蛋白脂肪酶活性，而通过常规测定方法在肝脏中未检测到该酶活性。2. 当由心脏和肝脏制备混合丙酮干粉时，预期活性受到明显抑制，表明肝脏中存在一种抑制剂。在存在相对大量肝素的情况下，这种抑制作用部分得到克服。3. 当向测定系统中加入大量肝素时，单独在肝脏中也检测到了脂蛋白脂肪酶。4. 给大鼠喂食脂肪餐后，未检测到肝脏或心脏中脂蛋白脂肪酶活性增加。5. 得出的结论是，大鼠肝脏含有通常处于无活性状态的脂蛋白脂肪酶。结果表明肝素酶是导致失活的因素。6. 讨论了肝脏中脂蛋白脂肪酶无功能状态的意义。结果支持这样一种观点，即肝脏直接水解血浆甘油三酯不是一个重要的生理过程。
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