甲状旁腺素的放射免疫测定。六种不同抗原和抗血清的特性

Radioimmunoassay for parathyrin. Characterization of six different antigens and antisera.

作者信息

Voll R, Schmidt-Gayk H, Wiedeman J, Hüfner M, Bouillon R, Keutmann H, Hehrmann R

出版信息

J Clin Chem Clin Biochem. 1978 May;16(5):269-77. doi: 10.1515/cclm.1978.16.5.269.

DOI:10.1515/cclm.1978.16.5.269

Abstract

We studied six different antisera to bovine or porcine parathyrin (parathyroid hormone, PTH), produced in rabbit, guinea pigs, sheep or goat, two of which are commercially available. The antisera were characterized with regard to species specificity, affinity and their ability to identify patients with primary hyperparathyroidism. In this heterologous radioimmunoassay system in which [125I]parathyrin is used as a tracer, some cross-reactivity of the antisera to the hormone or hormone fragments present in human serum was demonstrated. However, there is some overlap of serum immunoreactive parathyrin in patients with or without primary hyperparathyroidism. The results of this and other studies illustrate the necessity for a homologous radioimmunoassay for human parathyrin.

摘要

我们研究了六种针对牛或猪甲状旁腺素（甲状旁腺激素，PTH）的不同抗血清，这些抗血清由兔、豚鼠、绵羊或山羊产生，其中两种可商购。对这些抗血清在物种特异性、亲和力以及识别原发性甲状旁腺功能亢进患者的能力方面进行了表征。在这个以[125I]甲状旁腺素作为示踪剂的异源放射免疫分析系统中，证明了抗血清与人血清中存在的激素或激素片段有一些交叉反应。然而，原发性甲状旁腺功能亢进患者和非原发性甲状旁腺功能亢进患者的血清免疫反应性甲状旁腺素存在一些重叠。本研究及其他研究结果表明，需要一种针对人甲状旁腺素的同源放射免疫分析方法。