Hoss W, Smiley C
J Neurosci Res. 1977;3(4):249-56. doi: 10.1002/jnr.490030402.
The polarization of the native fluorescence of dopamine and noradrenaline has been used to measure their binding and immobilization by liposomes suspended in aqueous buffers. Whereas both catecholamines are significantly immobilized by brain phosphatidyl serine and yeast phosphatidyl inositol, phosphatidyl ethanolamine and phosphatidyl inositol from brain are ineffective. Dopamine is immobilized to a greater degree than noradrenaline. The dissociation constants determined from modified Scatchard plots of the polarization data are 1.7 X 10(-4) and 9.6 X 10(-5)M for dopamine with yeast phosphatidyl inositol and brain phosphatidyl serine, respectively. Apomorphine binds to a hydrophobic region of phosphatidyl serine liposomes with a KD value of 69 micrometer. It is suggested that a fraction of dopamine is complexed with membranous phosphatidyl serine in nerve terminals.
多巴胺和去甲肾上腺素的天然荧光偏振已被用于测量它们与悬浮在水性缓冲液中的脂质体的结合和固定情况。虽然这两种儿茶酚胺都能被脑磷脂酰丝氨酸和酵母磷脂酰肌醇显著固定，但脑磷脂酰乙醇胺和磷脂酰肌醇则没有效果。多巴胺的固定程度比去甲肾上腺素更高。根据偏振数据的修正Scatchard图确定，多巴胺与酵母磷脂酰肌醇和脑磷脂酰丝氨酸的解离常数分别为1.7×10⁻⁴和9.6×10⁻⁵M。阿扑吗啡以69微米的KD值与磷脂酰丝氨酸脂质体的疏水区域结合。有人提出，一部分多巴胺在神经末梢与膜性磷脂酰丝氨酸形成复合物。
