Qadri S M, Hoare D S
J Bacteriol. 1968 Jun;95(6):2344-57. doi: 10.1128/jb.95.6.2344-2357.1968.
A photosynthetic bacterium isolated by enrichment on media containing formate as major source of cell carbon was identified as a strain of Rhodopseudomonas palustris. It grew on a wide range of simple organic compounds including alcohols, fatty acids, and hydroxyacids, on a chemically defined medium with biotin and p-aminobenzoic acid as essential growth factors. The organism grew on formate or photoautotrophically with molecular hydrogen or thiosulfate only in the presence of yeast extract. Ability to photoassimilate formate could be shown only in organisms grown in the presence of formate. The organism contained an inducible formic hydrogenlyase consisting of a soluble formic dehydrogenase, a particulate hydrogenase, and one or more intermediate, but as yet unidentified, electron carriers. The formic hydrogenlyase could be reconstituted from a particulate hydrogenase and a partially purified soluble formic dehydrogenase. Some properties of the formic dehydrogenase and hydrogenase have been compared with that of the formic hydrogenlyase system.
通过在以甲酸盐作为细胞碳主要来源的培养基上富集培养而分离出的一种光合细菌，被鉴定为沼泽红假单胞菌菌株。它能在多种简单有机化合物上生长，包括醇类、脂肪酸和羟基酸，在一种以生物素和对氨基苯甲酸作为必需生长因子的化学限定培养基上也能生长。该生物体仅在酵母提取物存在的情况下，才能利用甲酸盐生长或以分子氢或硫代硫酸盐进行光自养生长。只有在甲酸盐存在下生长的生物体中，才能显示出光同化甲酸盐的能力。该生物体含有一种可诱导的甲酸氢化酶，它由一种可溶性甲酸脱氢酶、一种颗粒状氢化酶以及一种或多种尚未鉴定的中间电子载体组成。甲酸氢化酶可以由颗粒状氢化酶和部分纯化的可溶性甲酸脱氢酶重构而成。已将甲酸脱氢酶和氢化酶的一些特性与甲酸氢化酶系统的特性进行了比较。
