来自热醋酸梭菌的烟酰胺腺嘌呤二核苷酸磷酸依赖型甲酸脱氢酶的性质
Properties of Nicotinamide Adenine Dinucleotide Phosphate-Dependent Formate Dehydrogenase from Clostridium thermoaceticum.
作者信息
Li L F, Ljungdahl L, Wood H G
机构信息
Department of Biochemistry, Western Reserve University, School of Medicine, Cleveland, Ohio.
出版信息
J Bacteriol. 1966 Aug;92(2):405-12. doi: 10.1128/jb.92.2.405-412.1966.
Abstract
Li, Lan-Fun (Western Reserve University School of Medicine, Cleveland, Ohio), Lars Ljungdahl, and Harland G. Wood. Properties of nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase from Clostridium thermoaceticum. J. Bacteriol. 92: 405-412. 1966.-A nicotinamide adenine dinucleotide phosphate (NADP)-dependent formate dehydrogenase has been isolated from C. thermoaceticum. The enzyme is very sensitive to oxygen and requires sulfhydryl compounds for activity. The apparent K(m) at 50 C and pH 7.0 for NADP is 5.9 x 10(-5)m and for formate, 2.2 x 10(-4)m. The enzyme is most active at about 60 C and at pH values between 7.0 and 9.0. The enzyme catalyzes an exchange between C(14)O(2) and formate, which requires NADP, but net synthesis of formate from CO(2) and reduced nicotinamide adenine dinucleotide phosphate could not be demonstrated. The reaction does not involve ferredoxin.
摘要
李兰芬（俄亥俄州克利夫兰市西储大学医学院）、拉尔斯·永达尔和哈兰德·G·伍德。嗜热醋酸梭菌中烟酰胺腺嘌呤二核苷酸磷酸依赖性甲酸脱氢酶的性质。《细菌学杂志》92: 405 - 412。1966年。——已从嗜热醋酸梭菌中分离出一种烟酰胺腺嘌呤二核苷酸磷酸（NADP）依赖性甲酸脱氢酶。该酶对氧气非常敏感，其活性需要巯基化合物。在50℃和pH 7.0条件下，该酶对NADP的表观米氏常数（K(m)）为5.9×10⁻⁵m，对甲酸的表观米氏常数为2.2×10⁻⁴m。该酶在约60℃以及pH值在7.0至9.0之间时活性最高。该酶催化¹⁴CO₂与甲酸之间的交换反应，此反应需要NADP，但无法证明能从CO₂和还原型烟酰胺腺嘌呤二核苷酸磷酸净合成甲酸。该反应不涉及铁氧化还原蛋白。
相似文献
1
Properties of Nicotinamide Adenine Dinucleotide Phosphate-Dependent Formate Dehydrogenase from Clostridium thermoaceticum.来自热醋酸梭菌的烟酰胺腺嘌呤二核苷酸磷酸依赖型甲酸脱氢酶的性质
J Bacteriol. 1966 Aug;92(2):405-12. doi: 10.1128/jb.92.2.405-412.1966.
2
Nicotinamide adenine dinucleotide phosphate-dependent formate dehydrogenase from Clostridium thermoaceticum: purification and properties.来自热醋酸梭菌的烟酰胺腺嘌呤二核苷酸磷酸依赖性甲酸脱氢酶：纯化及性质
J Bacteriol. 1974 Oct;120(1):6-14. doi: 10.1128/jb.120.1.6-14.1974.
3
Formate dehydrogenase of Clostridium thermoaceticum: incorporation of selenium-75, and the effects of selenite, molybdate, and tungstate on the enzyme.热醋酸梭菌的甲酸脱氢酶：硒-75的掺入以及亚硒酸盐、钼酸盐和钨酸盐对该酶的影响。
J Bacteriol. 1973 Nov;116(2):867-73. doi: 10.1128/jb.116.2.867-873.1973.
4
INCORPORATION OF C14 FROM CARBON DIOXIDE INTO SUGAR PHOSPHATES, CARBOXYLIC ACIDS, AND AMINO ACIDS BY CLOSTRIDIUM THERMOACETICUM.热醋梭菌将二氧化碳中的碳-14掺入糖磷酸酯、羧酸和氨基酸的过程
J Bacteriol. 1965 Apr;89(4):1055-64. doi: 10.1128/jb.89.4.1055-1064.1965.
5
A hydrogen bond network in the active site of Anabaena ferredoxin-NADP(+) reductase modulates its catalytic efficiency.鱼腥藻铁氧化还原蛋白-NADP(+)还原酶活性位点中的氢键网络调节其催化效率。
Biochim Biophys Acta. 2014 Feb;1837(2):251-63. doi: 10.1016/j.bbabio.2013.10.010. Epub 2013 Nov 4.
6
Formate dehydrogenase from Clostridium acidiurici.来自尿酸梭菌的甲酸脱氢酶。
J Bacteriol. 1972 Jan;109(1):152-61. doi: 10.1128/jb.109.1.152-161.1972.
7
Interactions of nicotinamide-adenine dinucleotide phosphate analogues and fragments with pigeon liver malic enzyme. Synergistic effect between the nicotinamide and adenine moieties.烟酰胺腺嘌呤二核苷酸磷酸类似物及片段与鸽肝苹果酸酶的相互作用。烟酰胺与腺嘌呤部分之间的协同效应。
Biochem J. 1987 Jul 15;245(2):407-14. doi: 10.1042/bj2450407.
8
Fermentation of glucose, fructose, and xylose by Clostridium thermoaceticum: effect of metals on growth yield, enzymes, and the synthesis of acetate from CO 2 .热醋酸梭菌对葡萄糖、果糖和木糖的发酵：金属对生长产量、酶以及由二氧化碳合成乙酸的影响。
J Bacteriol. 1973 May;114(2):743-51. doi: 10.1128/jb.114.2.743-751.1973.
9
Purification and characterization of the two 6-phosphogluconate dehydrogenase species from Pseudomonas multivorans.多食假单胞菌中两种6-磷酸葡萄糖酸脱氢酶的纯化与特性分析
J Bacteriol. 1974 Dec;120(3):1043-57. doi: 10.1128/jb.120.3.1043-1057.1974.
10
A four-iron, four-sulfide ferredoxin with high thermostability from Clostridium thermoaceticum.来自热醋酸梭菌的具有高热稳定性的四铁四硫铁氧还蛋白。
J Bacteriol. 1977 Jun;130(3):1084-90. doi: 10.1128/jb.130.3.1084-1090.1977.
引用本文的文献
1
Four-electron reduction of CO: from formaldehyde and acetal synthesis to complex transformations.CO的四电子还原：从甲醛和缩醛合成到复杂转化
Chem Sci. 2024 Aug 5;15(37):15023-86. doi: 10.1039/d4sc02888k.
2
Isopropanol production via the thermophilic bioconversion of sugars and syngas using metabolically engineered Moorella thermoacetica.利用代谢工程改造的嗜热栖热放线菌通过糖类和合成气的嗜热生物转化生产异丙醇。
Biotechnol Biofuels Bioprod. 2024 Jan 28;17(1):13. doi: 10.1186/s13068-024-02460-1.
3
Flavin-Based Electron Bifurcation, Ferredoxin, Flavodoxin, and Anaerobic Respiration With Protons (Ech) or NAD (Rnf) as Electron Acceptors: A Historical Review.以黄素为基础的电子分叉、铁氧化还原蛋白、黄素氧化还原蛋白以及以质子（Ech）或NAD（Rnf）作为电子受体的无氧呼吸：历史回顾
Front Microbiol. 2018 Mar 14;9:401. doi: 10.3389/fmicb.2018.00401. eCollection 2018.
4
Biocatalysis for the application of CO2 as a chemical feedstock.用于将二氧化碳作为化学原料应用的生物催化。
Beilstein J Org Chem. 2015 Dec 1;11:2370-87. doi: 10.3762/bjoc.11.259. eCollection 2015.
5
A genome-guided analysis of energy conservation in the thermophilic, cytochrome-free acetogenic bacterium Thermoanaerobacter kivui.嗜热、无细胞色素产乙酸菌基维嗜热厌氧杆菌能量守恒的基因组导向分析。
BMC Genomics. 2014 Dec 18;15(1):1139. doi: 10.1186/1471-2164-15-1139.
6
Genome-wide effects of selenium and translational uncoupling on transcription in the termite gut symbiont Treponema primitia.全基因组范围内硒和翻译解偶联对原生质体肠道共生菌转录的影响。
mBio. 2013 Nov 12;4(6):e00869-13. doi: 10.1128/mBio.00869-13.
7
Acetogenesis and the Wood-Ljungdahl pathway of CO(2) fixation.产乙酸作用与二氧化碳固定的伍德-Ljungdahl途径。
Biochim Biophys Acta. 2008 Dec;1784(12):1873-98. doi: 10.1016/j.bbapap.2008.08.012. Epub 2008 Aug 27.
8
The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum).嗜热栖热菌（原热醋梭菌）的全基因组序列
Environ Microbiol. 2008 Oct;10(10):2550-73. doi: 10.1111/j.1462-2920.2008.01679.x. Epub 2008 Jun 9.
9
Total synthesis of acetate from CO2. II. Purification and properties of formyltetrahydrofolate synthetase from Clostridium thermoaceticum.
J Bacteriol. 1969 May;98(2):842-4. doi: 10.1128/jb.98.2.842-844.1969.
10
[CO2 fixation by Clostridium aceticum: short-term 14CO2 incorporation and pyruvate metabolism].[醋酸梭菌的二氧化碳固定作用：短期14CO2掺入及丙酮酸代谢]
Arch Mikrobiol. 1969;64(3):203-14.
本文引用的文献
1
The manometric determination of formic acid.甲酸的压力测定法。
Biochem J. 1946;40(1):100-2. doi: 10.1042/bj0400100.
2
Carbon Dioxide Utilization in the Synthesis of Acetic Acid by Clostridium Thermoaceticum.嗜热醋酸梭菌利用二氧化碳合成乙酸
Proc Natl Acad Sci U S A. 1945 Aug;31(8):219-25. doi: 10.1073/pnas.31.8.219.
3
A study of carbon dioxide fixation by mass determination of the types of C13-acetate.通过对 C13 - 醋酸盐类型进行质量测定来研究二氧化碳固定作用。
J Biol Chem. 1952 Feb;194(2):905-31.
4
The reaction of pyridine nucleotide with cyanide and its analytical use.吡啶核苷酸与氰化物的反应及其分析应用。
J Biol Chem. 1951 Aug;191(2):447-59.
5
An electron transport factor from Clostridium pasteurianum.来自巴氏梭菌的一种电子传递因子。
Biochem Biophys Res Commun. 1962 Jun 4;7:448-52. doi: 10.1016/0006-291x(62)90333-9.
6
PYRUVATE SYNTHESIS BY A PARTIALLY PURIFIED ENZYME FROM CLOSTRIDIUM ACIDI-URICI.尿酸梭菌中部分纯化酶催化的丙酮酸合成
Biochem Biophys Res Commun. 1965 Feb 3;18:303-7. doi: 10.1016/0006-291x(65)90703-5.
7
INCORPORATION OF C14 FROM CARBON DIOXIDE INTO SUGAR PHOSPHATES, CARBOXYLIC ACIDS, AND AMINO ACIDS BY CLOSTRIDIUM THERMOACETICUM.热醋梭菌将二氧化碳中的碳-14掺入糖磷酸酯、羧酸和氨基酸的过程
J Bacteriol. 1965 Apr;89(4):1055-64. doi: 10.1128/jb.89.4.1055-1064.1965.
8
BACTERIAL FERREDOXIN.细菌铁氧化还原蛋白
Bacteriol Rev. 1964 Dec;28(4):497-517. doi: 10.1128/br.28.4.497-517.1964.
9
ANAEROBIC FORMATE OXIDATION: A FERREDOXIN-DEPENDENT REACTION.厌氧甲酸氧化：一种依赖铁氧化还原蛋白的反应。
Science. 1964 Apr 17;144(3616):297-8. doi: 10.1126/science.144.3616.297.
10
FERREDOXIN AS A REDUCTANT IN PYRUVATE SYNTHESIS BY A BACTERIAL EXTRACT.铁氧化还原蛋白作为细菌提取物合成丙酮酸的还原剂
Proc Natl Acad Sci U S A. 1964 Apr;51(4):690-4. doi: 10.1073/pnas.51.4.690.
Zotero 插件