Sapozhnikova L R, Katinas G S, Lonshakov S V
Arkh Anat Gistol Embriol. 1978 Apr;74(4):69-73.
The course of mitotic activity in the rabbit mesothelium of parietal peritoneum was studied depending upon the daytime when the lesion was produced. In the first series of experiments (I) the operation was performed from 8 to 10 a. m., and in the second series (II) - from 8 to 10 P. M. After the operation the animals were sacrifieced: one animal every 3 h during 5 days. Mitotic activity was investigated along 13 mm from the edge of the wound in flat film preparations. The data obtained were statistically treated applying approximation of the process course by the method of weighed sliding averages. The first mesothelial mitoses appeared: in the first series (I) of experiments - in 24 h, in the second series (II) of experiments - 21 h. Further, proliferation of the mesothelium appeared in different parts of the tissue with various intencity and was of wavy character. Regardless of the time when the lesion was produced, mitotic activity was the greatest in the first zone, 4.8 mm wide its maximum 33 h after the operation. The character of the regenerative process in the mesothelium depends on the circadian phase of the organism at the time of the operation, that influences the time when mitotic activity begins, its quantitative indices, the area of the tissue involving into the reaction, expressiveness of the rhythmicity of the process. During 5 days, in the II series of experiments three waves were noted, and in the I series - five waves were noted and their periods were near the diurnal.
根据产生损伤的日间时间，研究了家兔壁腹膜间皮的有丝分裂活动过程。在第一组实验（I）中，手术于上午8点至10点进行，在第二组实验（II）中，手术于晚上8点至10点进行。手术后，在5天内每3小时处死1只动物。在平板制片中，沿着距伤口边缘13毫米的区域研究有丝分裂活动。对所得数据应用加权滑动平均法对过程进行近似处理后进行统计学分析。首次出现间皮有丝分裂：在第一组实验（I）中为术后24小时，在第二组实验（II）中为术后21小时。此外，间皮在组织的不同部位以不同强度出现增殖，且呈波浪状。无论产生损伤的时间如何，有丝分裂活动在第一个区域最大，该区域宽4.8毫米，术后33小时达到最大值。间皮再生过程的特征取决于手术时机体的昼夜节律阶段，这会影响有丝分裂活动开始的时间、其定量指标、参与反应的组织面积以及过程节律的表现程度。在5天内，在第二组实验中观察到3个波，在第一组实验中观察到5个波，且它们的周期接近昼夜节律。
