Spielmann H, Eibs H G, Jacob-Müller U, Bischoff R
Biochem Genet. 1978 Apr;16(3-4):191-202. doi: 10.1007/BF00484077.
Extensive extraction studies with Triton X-100 revealed only LDH-1 (B4) but no trace of LDH-5 (A4) in one-cell and two-cell mouse and rat embryos. The LDH isozyme pattern of preimplantation mouse embryos changes from the maternally inherited B subunit isozyme (LDH-1) to a pattern dominated by LDH-5 when mouse blastocysts are cultured under conditions that prevent hatching but allow trophoblast giant cell transformation. During differentiation of mouse blastocysts in vitro, implantation is therefore not essential for the appearance of the A subunit form of LDH (LDH-5) coded for by the embryonic genome. Mechanisms controlling the expression of LDH-5 in mouse blastocysts during in vivo development are discussed.
用曲拉通X - 100进行的广泛提取研究表明，在单细胞和双细胞小鼠及大鼠胚胎中仅发现了乳酸脱氢酶 - 1（B4），未检测到乳酸脱氢酶 - 5（A4）的踪迹。当小鼠囊胚在防止孵化但允许滋养层巨细胞转化的条件下培养时，植入前小鼠胚胎的乳酸脱氢酶同工酶模式从母系遗传的B亚基同工酶（乳酸脱氢酶 - 1）转变为以乳酸脱氢酶 - 5为主导的模式。因此，在小鼠囊胚体外分化过程中，植入对于胚胎基因组编码的乳酸脱氢酶A亚基形式（乳酸脱氢酶 - 5）的出现并非必不可少。本文讨论了在体内发育过程中控制小鼠囊胚中乳酸脱氢酶 - 5表达的机制。
