[造血器官的基质成纤维细胞与培养中的抗体形成]
[Stromal fibroblasts of the hematopoietic organs and antibody formation in cultures].
作者信息
Sidorenko A V, Kulagina N N
出版信息
Biull Eksp Biol Med. 1978;86(7):49-51.
The thymus, bone marrow- and spleen-derived stromal mechanocytes from the monolayer cultures (3rd--6th passages) when added to the suspension cultures of rabbit spleen cells according to Mishell and Dutton produced a considerable effect on the plaque-forming cells (PFC) accumulation by the 4th day of cultivation. Their action distinctly depended on the dose. Bone marrow-derived stromal mechanocytes in doses of 2.1 X 10(3)--6.25 X 10(5) caused inhibition of PFC formation in cultures. Thymus-derived stromal mechanocytes in doses of 2.75 X 10(3)--8 X 10(5) cause an increase in number of PFC; spleen-derived stromal mechanocytes in doses of 2.1 X 10(3)--1.3 X 10(4) failed to bring about any significant changes, but when the dose was increased to 8 X 10(4)--6.25 X 10(5) the inhibition of PFC formation took place. Most of the live cells and PFC were found in the free cells fraction.
将单层培养（第3 - 6代）的胸腺、骨髓和脾脏来源的基质机械细胞，按照米舍尔和达顿的方法加入兔脾细胞悬浮培养物中，到培养第4天时，对空斑形成细胞（PFC）的积累产生了显著影响。它们的作用明显取决于剂量。剂量为2.1×10³ - 6.25×10⁵的骨髓来源的基质机械细胞会抑制培养物中PFC的形成。剂量为2.75×10³ - 8×10⁵的胸腺来源的基质机械细胞会使PFC数量增加；剂量为2.1×10³ - 1.3×10⁴的脾脏来源的基质机械细胞未引起任何显著变化，但当剂量增加到8×10⁴ - 6.25×10⁵时，PFC的形成受到抑制。大部分活细胞和PFC存在于游离细胞部分。
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