Latsinik N V, Sidorovich S I
Biull Eksp Biol Med. 1978 Aug;86(8):216-8.
A method of removal of phagocytising elements from the cell suspension by means of iron powder in magnetic field was used. Clonogenic fibroblast precursors of the hemopoietic tissue not belonging to histiocytes-macrophages, but referred to mechanocytes, were characterised by high phagocytic activity. Following Fe treatment less than 1% of clonogenic fibroblast precursors which gave rise to fibroblast colonies under conditions of monolayer cultures remained in the bone marrow cell suspension, and about 10%--in the spleen and the peritoneal exudate cell suspension.
采用了一种通过在磁场中利用铁粉从细胞悬液中去除吞噬细胞成分的方法。造血组织中不属于组织细胞 - 巨噬细胞而被称为机械细胞的克隆形成性成纤维细胞前体具有高吞噬活性。经铁处理后，在单层培养条件下能形成成纤维细胞集落的克隆形成性成纤维细胞前体，在骨髓细胞悬液中残留不到1%，在脾脏和腹腔渗出液细胞悬液中约为10%。
