Kulagina N N, Sidorenko A V
Biull Eksp Biol Med. 1978 Jun;85(6):699-701.
Stromal fibroblasts from the monolayer cultures of the rabbit thymus, spleen, and bone marrow when added to suspension cultures of non-adherent rabbit spleen cells had a significant effect on the response of the plaque-forming cells (PFC). Thymus-derived stromal fibroblasts caused an increase in the number of PFC, and bone marrow stromal fibroblasts suppressed the antibody formation in these cultures. Spleen-derived stromal fibroblasts influenced the PFC response similarly to adherent cells. Thymus-derived stromal fibroblasts irradiated by 5000 R influenced the PFC response in the same way as non-irradiated cells. The action of stromal fibroblasts on the antibody formation in the cultures depended on their attachment to the culture flask surface.
将来自兔胸腺、脾脏和骨髓单层培养物的基质成纤维细胞添加到非贴壁兔脾细胞的悬浮培养物中时，对空斑形成细胞（PFC）的反应有显著影响。胸腺来源的基质成纤维细胞导致PFC数量增加，而骨髓基质成纤维细胞抑制这些培养物中的抗体形成。脾脏来源的基质成纤维细胞对PFC反应的影响与贴壁细胞类似。经5000伦琴照射的胸腺来源的基质成纤维细胞对PFC反应的影响与未照射的细胞相同。基质成纤维细胞对培养物中抗体形成的作用取决于它们与培养瓶表面的附着。
