Brady P S, Brady L J, Whetter P A, Ullrey D E, Fay L D
J Nutr. 1978 Sep;108(9):1439-48. doi: 10.1093/jn/108.9.1439.
Thirty-two adult female white-tailed deer were assigned to four complete pelleted diets (+/- 45 ppm vitamin E; +/- 0.2 ppm selenium). Selenium and vitamin E concentration in the unsupplemented diet was 0.04 and 5.5 ppm, respectively. Biochemical parameters of the erythrocyte (RBC) glutathione peroxidase system and survival of off-spring to weaning were followed for 2 years. At the end of the second year, 12 male young (3 per treatment) and the remaining adults were killed, and liver and muscle parameters of the glutathione system determined. Plasma selenium (Se) and vitamin E (E) were significantly lower among unsupplemented adults within 6 months of treatment and remained essentially constant from 10 months on. In vitro hemolysis and mortality of young were affected by dietary E but not by Se. Tissue glutathione peroxidase (GSH-Px) was correlated with tissue Se in all tissues measured (RBC, liver and muscle). Tissue Se, in turn, was related to dietary Se. Thus, dietary Se deficiency (Se = 0.04 ppm) resulted in biochemical deficiency (depressed GSH-Px). This was not reflected in gross lesions among the adults, nor in increased mortality among young.
32只成年雌性白尾鹿被分配到四种完全颗粒饲料组（维生素E含量±45 ppm；硒含量±0.2 ppm）。未添加组饲料中硒和维生素E的浓度分别为0.04 ppm和5.5 ppm。对红细胞（RBC）谷胱甘肽过氧化物酶系统的生化参数以及后代存活至断奶的情况进行了为期2年的跟踪。在第二年年底，处死了12只雄性幼鹿（每组处理3只）和其余的成年鹿，并测定了谷胱甘肽系统的肝脏和肌肉参数。在处理后的6个月内，未添加组成年鹿的血浆硒（Se）和维生素E（E）显著降低，从10个月起基本保持恒定。幼鹿的体外溶血和死亡率受饲料中维生素E的影响，但不受硒的影响。在所测量的所有组织（红细胞、肝脏和肌肉）中，组织谷胱甘肽过氧化物酶（GSH-Px）与组织硒相关。反过来，组织硒又与饲料中的硒相关。因此，饲料硒缺乏（硒含量=0.04 ppm）导致生化缺乏（谷胱甘肽过氧化物酶降低）。这在成年鹿中未表现为明显病变，在幼鹿中也未导致死亡率增加。
