Chow C K, Chen C J
J Nutr. 1980 Dec;110(12):2460-6. doi: 10.1093/jn/110.12.2460.
The effects of age and dietary selenium on cellular susceptibility to oxidative stress were studied in rat erythrocytes. Young (26 or 40 days) and mature adult (11 or 15 months) male rats were fed a Torula yeast-based low selenium and low vitamin E diet supplemented with either 0, 1.0 to 2.0 ppm selenium (as sodium selenite) for 5 weeks. The rates of spontaneous hemolysis amd methemoglobin formation and levels of glutathione (GSH), glucose-6-phosphate (G6P) dehydrogenase, but not of GSH peroxidase, GSH reductase, superoxide dismutase and catalase were significantly higher in the erythrocytes of young rats than in those of the adults. Adult rats, however, had higher levels of plasma vitamin E and GSH peroxidase than those of the young group. Dietary selenium markedly increased activity of plasma GSH peroxidase and partially retarded the rates of hemolysis and methomoglobin formation, but it had no significant effect on the erythrocyte levels of GSH, GSH reductase, catalse, G6P dehydrogenase and superoxide dismutase or plasma vitamin E levels of young rats. Except of GSH peroxidase activity, dietary selenium had no significant effect on any other measurements made in the erythrocytes or plasma of adult rats. The results suggest that the ability of adult rats. The results suggest that the ability of adult rats to retain higher levels of vitamin E and selenium than the young rats may be partly responsible for the increased resistance of their erythrocytes against oxidative stress.
在大鼠红细胞中研究了年龄和膳食硒对细胞氧化应激易感性的影响。将年轻（26或40天）和成年（11或15个月）雄性大鼠喂食以产朊假丝酵母为基础的低硒和低维生素E饮食，并补充0、1.0至2.0 ppm硒（以亚硒酸钠形式），持续5周。年轻大鼠红细胞中的自发溶血和高铁血红蛋白形成速率以及谷胱甘肽（GSH）、葡萄糖-6-磷酸（G6P）脱氢酶的水平，但不包括GSH过氧化物酶、GSH还原酶、超氧化物歧化酶和过氧化氢酶的水平，显著高于成年大鼠。然而，成年大鼠的血浆维生素E和GSH过氧化物酶水平高于年轻组。膳食硒显著提高了血浆GSH过氧化物酶的活性，并部分延缓了溶血和高铁血红蛋白形成的速率，但对年轻大鼠红细胞中GSH、GSH还原酶、过氧化氢酶、G6P脱氢酶和超氧化物歧化酶的水平或血浆维生素E水平没有显著影响。除了GSH过氧化物酶活性外，膳食硒对成年大鼠红细胞或血浆中的任何其他测量指标均无显著影响。结果表明，成年大鼠比年轻大鼠能够保留更高水平的维生素E和硒，这可能部分是其红细胞对氧化应激抵抗力增强的原因。
