豚鼠血清中IgG、IgM和B1C球蛋白对肠炎沙门氏菌的黏附作用
The adherence to Salmonella enteritidis of IgG, IgM and B1C-Globulins from guinea-pig serum.
作者信息
Beernink K D, Steward J P
出版信息
Immunology. 1968 Aug;15(2):319-21.
The adherence of globulins from guinea-pig serum to was determined when these bacteria were sensitized : (a) in serum from normal guinea-pigs lacking detectable bactericidal antibody, (b) in serum from normal guinea-pigs possessing low titre of bactericidal antibody, and (c) in serum from guinea-pigs actively immunized against this . Sensitized bacteria were washed free of contaminating serum constituents and used to immunize rabbits. Guinea-pig globulins that adhered to the bacteria and to which the rabbit responded by the production of antibody were identified by immunoelectrophoresis. IgM, but no IgG, attached to bacteria sensitized in serum lacking bactericidins. Both IgG and IgM attached to bacteria sensitized in immune serum in serum containing `normal' bactericidins. β-globulin attached to bacteria in all three cases.
当这些细菌被致敏时，测定了豚鼠血清中球蛋白与以下情况的结合：(a) 来自缺乏可检测杀菌抗体的正常豚鼠的血清；(b) 来自具有低滴度杀菌抗体的正常豚鼠的血清；(c) 来自主动免疫该细菌的豚鼠的血清。将致敏细菌洗涤以去除污染的血清成分，并用其免疫兔子。通过免疫电泳鉴定与细菌结合且兔子通过产生抗体做出反应的豚鼠球蛋白。在缺乏杀菌素的血清中致敏的细菌上附着有IgM，但没有IgG。在含有“正常”杀菌素的免疫血清中致敏的细菌上同时附着有IgG和IgM。在所有三种情况下，β球蛋白都附着在细菌上。
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