分解代谢类固醇和合成代谢类固醇对骨骼肌核糖体氨基酸掺入的影响。
The effects of catabolic and anabolic steroids on amino acid incorporation by skeletal-muscle ribosomes.
作者信息
Bullock G, White A M, Worthington J
出版信息
Biochem J. 1968 Jul;108(3):417-25. doi: 10.1042/bj1080417.
Abstract
- A method is described for the routine isolation of ribosomes from small quantities of skeletal muscle that have been homogenized with the Ultra-Turrax tissue disintegrator. 2. Ribosomes prepared by this method from rats receiving triamcinolone acetonide or rabbits receiving cortisone acetate show a marked fall in their ability to incorporate amino acids when compared with ribosomes from control animals. 3. This fall in activity can be partially prevented in rats by pretreating the animals with an anabolic steroid, steroid 36644-Ba. 4. Testosterone (5mg./kg.) administered to rabbits in conjunction with cortisone acetate is not effective in maintaining ribosomal activity. However, steroid 36644-Ba at one-tenth of an equiandrogenic dose (0.05mg./kg.) is extremely effective. 5. The results with ribosomes isolated from rabbits support the concept that steroid 36644-Ba and possibly all anabolic steroids have an ability to counteract the catabolic action of corticosteroids that is greater than their androgenic activity would suggest.
摘要
- 本文描述了一种从用Ultra - Turrax组织捣碎机匀浆后的少量骨骼肌中常规分离核糖体的方法。2. 用这种方法从接受曲安奈德的大鼠或接受醋酸可的松的兔子制备的核糖体，与对照动物的核糖体相比，其掺入氨基酸的能力显著下降。3. 通过用合成代谢类固醇36644 - Ba预处理动物，大鼠核糖体活性的这种下降可得到部分预防。4. 给兔子注射睾酮（5毫克/千克）并同时给予醋酸可的松，对维持核糖体活性无效。然而，合成代谢类固醇36644 - Ba以等效雄激素剂量的十分之一（0.05毫克/千克）给药则极为有效。5. 从兔子分离的核糖体的实验结果支持了这样一种观点，即合成代谢类固醇36644 - Ba以及可能所有的合成代谢类固醇，都具有抵消皮质类固醇分解代谢作用的能力，且这种能力大于其雄激素活性所表明的。
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