分解代谢类固醇和合成代谢类固醇对骨骼肌核糖体氨基酸掺入的影响。
The effects of catabolic and anabolic steroids on amino acid incorporation by skeletal-muscle ribosomes.
作者信息
Bullock G, White A M, Worthington J
出版信息
Biochem J. 1968 Jul;108(3):417-25. doi: 10.1042/bj1080417.
Abstract
- A method is described for the routine isolation of ribosomes from small quantities of skeletal muscle that have been homogenized with the Ultra-Turrax tissue disintegrator. 2. Ribosomes prepared by this method from rats receiving triamcinolone acetonide or rabbits receiving cortisone acetate show a marked fall in their ability to incorporate amino acids when compared with ribosomes from control animals. 3. This fall in activity can be partially prevented in rats by pretreating the animals with an anabolic steroid, steroid 36644-Ba. 4. Testosterone (5mg./kg.) administered to rabbits in conjunction with cortisone acetate is not effective in maintaining ribosomal activity. However, steroid 36644-Ba at one-tenth of an equiandrogenic dose (0.05mg./kg.) is extremely effective. 5. The results with ribosomes isolated from rabbits support the concept that steroid 36644-Ba and possibly all anabolic steroids have an ability to counteract the catabolic action of corticosteroids that is greater than their androgenic activity would suggest.
摘要
- 本文描述了一种从用Ultra - Turrax组织捣碎机匀浆后的少量骨骼肌中常规分离核糖体的方法。2. 用这种方法从接受曲安奈德的大鼠或接受醋酸可的松的兔子制备的核糖体，与对照动物的核糖体相比，其掺入氨基酸的能力显著下降。3. 通过用合成代谢类固醇36644 - Ba预处理动物，大鼠核糖体活性的这种下降可得到部分预防。4. 给兔子注射睾酮（5毫克/千克）并同时给予醋酸可的松，对维持核糖体活性无效。然而，合成代谢类固醇36644 - Ba以等效雄激素剂量的十分之一（0.05毫克/千克）给药则极为有效。5. 从兔子分离的核糖体的实验结果支持了这样一种观点，即合成代谢类固醇36644 - Ba以及可能所有的合成代谢类固醇，都具有抵消皮质类固醇分解代谢作用的能力，且这种能力大于其雄激素活性所表明的。
相似文献
1
The effects of catabolic and anabolic steroids on amino acid incorporation by skeletal-muscle ribosomes.分解代谢类固醇和合成代谢类固醇对骨骼肌核糖体氨基酸掺入的影响。
Biochem J. 1968 Jul;108(3):417-25. doi: 10.1042/bj1080417.
2
Relative changes in the function of muscle ribosomes and mitochondria during the early phase of steroid-induced catabolism.类固醇诱导分解代谢早期肌肉核糖体和线粒体功能的相对变化。
Biochem J. 1972 May;127(5):881-92. doi: 10.1042/bj1270881.
3
Some biochemical effects of triamcinolone acetonide on rat liver and muscle.曲安奈德对大鼠肝脏和肌肉的一些生化作用。
Biochem J. 1970 Feb;116(3):349-55. doi: 10.1042/bj1160349.
4
[Ribosomes of the mammary gland. Isolation and the capacity to incorporate in vitro amino acids into proteins].[乳腺核糖体。体外分离及将氨基酸掺入蛋白质的能力]
Bull Soc Chim Biol (Paris). 1968 Nov 5;50(7):1273-89.
5
[Action of anabolic steroids on the development of the genital system in the rabbit. VI. Anabolic and androgenic activity of 13 beta-ethyl-17 beta-hydroxy-17 alpha-ethyl-4-gonen-3-one (Norboletone) in the rat].[同化类固醇对家兔生殖系统发育的作用。VI. 13β-乙基-17β-羟基-17α-乙基-4-雄烯-3-酮（诺勃酮）在大鼠体内的同化及雄激素活性]
Boll Soc Ital Biol Sper. 1968 Aug 31;44(16):1310-3.
6
Changes in mitochondrial structure and ribosomal activity in muscle as a consequence of the interaction between a glucocorticoid and some anabolic steroids.糖皮质激素与某些合成代谢类固醇相互作用导致的肌肉中线粒体结构和核糖体活性的变化。
Biochem J. 1969 Dec;115(5):47P. doi: 10.1042/bj1150047pa.
7
Metabolic effects of anabolic steroid on skeletal muscle.合成代谢类固醇对骨骼肌的代谢影响。
Med Sci Sports. 1979 Summer;11(2):160-3.
8
In vitro amino acid incorporation into myosin by free polysomes of rat skeletal muscle.
Biochem Biophys Res Commun. 1971 Jun 4;43(5):1139-44. doi: 10.1016/0006-291x(71)90581-x.
9
Interaction between catabolic and anabolic steroids.分解代谢类固醇与合成代谢类固醇之间的相互作用。
Curr Ther Res Clin Exp. 1970 Oct;12(10):658-76.
10
Rapid mitochondrial enlargement in muscle as a response to triamcinolone acetonide and its relationship to the ribosomal defect.肌肉中作为对曲安奈德的反应的快速线粒体增大及其与核糖体缺陷的关系。
Biochem Pharmacol. 1971 May;20(5):943-53. doi: 10.1016/0006-2952(71)90314-5.
引用本文的文献
1
Experimental corticosteroid myopathy.实验性皮质类固醇肌病
Acta Neuropathol. 1981;55(3):167-72. doi: 10.1007/BF00691314.
2
Effects of a mild weight-lifting program on the progress of glucocorticoid-induced atrophy in rat hindlimb muscles.轻度举重训练计划对糖皮质激素诱导的大鼠后肢肌肉萎缩进程的影响。
Pflugers Arch. 1980 May;385(2):147-53. doi: 10.1007/BF00588695.
3
Changes in prostaglandin release associated with inhibition of muscle protein synthesis by dexamethasone.与地塞米松抑制肌肉蛋白质合成相关的前列腺素释放变化。
Biochem J. 1984 May 1;219(3):953-7. doi: 10.1042/bj2190953.
4
Intracellular enzymes and protein synthesis in rabbit skin after thermal injury.热损伤后兔皮肤中的细胞内酶与蛋白质合成
Br J Pharmacol. 1971 Jul;42(3):437-46. doi: 10.1111/j.1476-5381.1971.tb07129.x.
5
Activity of skeletal-muscle ribosomes from intact and castrated rats treated with triamcinolone acetonide.用曲安奈德处理的完整大鼠和去势大鼠骨骼肌核糖体的活性。
Biochem J. 1970 Jun;118(2):30P-31P. doi: 10.1042/bj1180030pb.
6
Some biochemical effects of triamcinolone acetonide on rat liver and muscle.曲安奈德对大鼠肝脏和肌肉的一些生化作用。
Biochem J. 1970 Feb;116(3):349-55. doi: 10.1042/bj1160349.
7
Changes in mitochondrial structure and ribosomal activity in muscle as a consequence of the interaction between a glucocorticoid and some anabolic steroids.糖皮质激素与某些合成代谢类固醇相互作用导致的肌肉中线粒体结构和核糖体活性的变化。
Biochem J. 1969 Dec;115(5):47P. doi: 10.1042/bj1150047pa.
8
Protein synthesis by membrane-bound and free ribosomes of secretory and non-secretory tissues.分泌性和非分泌性组织的膜结合核糖体和游离核糖体的蛋白质合成。
Biochem J. 1971 Feb;121(4):683-94. doi: 10.1042/bj1210683.
9
Relative changes in the function of muscle ribosomes and mitochondria during the early phase of steroid-induced catabolism.类固醇诱导分解代谢早期肌肉核糖体和线粒体功能的相对变化。
Biochem J. 1972 May;127(5):881-92. doi: 10.1042/bj1270881.
10
本文引用的文献
1
High-Resolution Density Gradient Sedimentation Analysis.高分辨率密度梯度沉降分析。
Science. 1960 Jan 1;131(3392):32-3. doi: 10.1126/science.131.3392.32.
2
The incorporation of [C]leucine into serum albumin by the isolated microsome fraction from rat liver.大鼠肝脏分离的微粒体部分将[C]亮氨酸掺入血清白蛋白的过程。
Biochem J. 1962 Feb;82(2):262-6. doi: 10.1042/bj0820262.
3
4
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
5
The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides.大肠杆菌中无细胞蛋白质合成对天然存在或合成的多聚核糖核苷酸的依赖性。
Proc Natl Acad Sci U S A. 1961 Oct 15;47(10):1588-602. doi: 10.1073/pnas.47.10.1588.
6
Preparation and amino acid incorporating ability of ribonucleoprotein-particles from different tissues of the rat.大鼠不同组织核糖核蛋白颗粒的制备及其氨基酸掺入能力
Exp Cell Res. 1960 Mar;19:253-66. doi: 10.1016/0014-4827(60)90006-9.
7
The effect of growth hormone and of cortisol on the response of isolated rat diaphragm to the stimulating effect of insulin on glucose uptake and on incorporation of amino acids into protein.生长激素和皮质醇对离体大鼠膈肌对胰岛素刺激葡萄糖摄取及氨基酸掺入蛋白质作用的反应的影响。
J Endocrinol. 1959 Jul;18:395-408. doi: 10.1677/joe.0.0180395.
8
9
AMINO ACID INCORPORATION INTO PROTEIN BY CELL-FREE PREPARATIONS FROM RAT SKELETAL MUSCLE. II. PREPARATION AND PROPERTIES OF MUSCLE RIBOSOMES AND POLYRIBOSOMES.大鼠骨骼肌无细胞制剂将氨基酸掺入蛋白质。II. 肌肉核糖体和多核糖体的制备及特性
Biochemistry. 1964 Nov;3:1713-9. doi: 10.1021/bi00899a020.
10
EFFECTS OF CORTISONE ON IN VITRO INCORPORATION OF GLYCINE INTO PROTEIN OF RAT DIAPHRAGM.可的松对甘氨酸在大鼠膈肌蛋白质中体外掺入的影响。
Endocrinology. 1964 May;74:709-13. doi: 10.1210/endo-74-5-709.
Zotero 插件