Gupta U S
Biochem Exp Biol. 1977;13(2):147-57.
The haemolymph proteins of the 6th nymph of P. picta were fractioned by the polyacrylamide gel disc electrophoresis. A total of seven proteins fractions have been detected from the haemolymph. The chemical nature of different protein fractions have been examined by histochemical methods. The changes taking place in the cuticle and epidermal cells have been examined during the transformation of 6th nymph into adult. The fat body proteins have been electrophoretically fractioned and the changes in the concentration of different protein fractions have been examined. It is suggested that the protein fraction 3 of the haemolymph is utilized in the formation of new cuticle. It is concluded by the histochemical observations that proteins of the band 3 are synthesized in the fat body.
采用聚丙烯酰胺凝胶圆盘电泳法对彩纹蝽若虫6龄若虫的血淋巴蛋白进行了分级分离。从血淋巴中共检测到7种蛋白组分。采用组织化学方法研究了不同蛋白组分的化学性质。在6龄若虫向成虫转变过程中,观察了角质层和表皮细胞的变化。对脂肪体蛋白进行了电泳分级,并检测了不同蛋白组分浓度的变化。结果表明,血淋巴中的蛋白组分3被用于新角质层的形成。组织化学观察结果表明,带3的蛋白是在脂肪体中合成的。