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[用等电聚焦法研究鸡和小鼠干扰素]

[Study of chicken and mouse interferons by the isoelectric focusing method].

作者信息

Zaretskiĭ I Z, Petukhova M B, Ershov F I

出版信息

Biull Eksp Biol Med. 1978 Aug;86(8):184-7.

PMID:567506
Abstract

Results of investigation of the chicken and mouse interferons by electrofocussing in sucrose and in polyacrylamide gel are described. The isoelectric points of these interferons were determined. The advantages of the electrofocussing in gel over that in sucrose for the interferon analysis was shown.

摘要

本文描述了通过在蔗糖和聚丙烯酰胺凝胶中进行电聚焦对鸡和小鼠干扰素的研究结果。测定了这些干扰素的等电点。结果表明,与在蔗糖中进行电聚焦相比,在凝胶中进行电聚焦用于干扰素分析具有优势。

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1
[Study of chicken and mouse interferons by the isoelectric focusing method].[用等电聚焦法研究鸡和小鼠干扰素]
Biull Eksp Biol Med. 1978 Aug;86(8):184-7.
2
Extraction of proteins within ultrathin-layer polyacrylamide electrophoresis (SDS-PAGE) and isoelectric focusing (PAGIF) of cryostat sections and tissue culture specimens.在低温恒温器切片和组织培养标本的超薄层聚丙烯酰胺电泳(SDS-PAGE)和等电聚焦(PAGIF)中提取蛋白质。
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Purification of human leukocyte interferon by two-dimensional polyacrylamide gel electrophoresis.通过二维聚丙烯酰胺凝胶电泳纯化人白细胞干扰素。
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J Gen Virol. 1978 Apr;39(1):125-30. doi: 10.1099/0022-1317-39-1-125.
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