Watson J V, Chambers S H
Cell Tissue Kinet. 1978 Jul;11(4):415-22. doi: 10.1111/j.1365-2184.1978.tb00813.x.
Simultaneous RNA and DNA estimations were carried out during the cell cycle of EMT6/M/CC cells growing in vitro following synchronization by mitotic selection. The determinations were performed with a flow cytofluorimeter on individual cells stained with acridine orange. It was found that the RNA content increased during G1 then remained virtually constant between early and mid S phase, but a second increase occurred during late S. The rate of uptake of tritiated uridine paralleled these changes in RNA levels, and it was also found that the rate of uptake in metaphase and anaphase was virtually zero, but a rapid increase occurred in telophase. The increase in DNA during S was approximately linear, and the intermitotic phase and cycle durations were very similar to previously reported results.
在通过有丝分裂选择同步化后，对体外生长的EMT6/M/CC细胞的细胞周期进行了RNA和DNA的同步测定。使用流式细胞荧光仪对用吖啶橙染色的单个细胞进行测定。发现RNA含量在G1期增加，然后在S期早期和中期基本保持恒定，但在S期后期出现第二次增加。氚标记尿苷的摄取速率与RNA水平的这些变化平行，并且还发现中期和后期的摄取速率几乎为零，但在末期迅速增加。S期DNA的增加大致呈线性，有丝分裂间期和细胞周期持续时间与先前报道的结果非常相似。
