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分离叶绿体中叶绿体DNA的合成。

Synthesis of chloroplast DNA in isolated chloroplasts.

作者信息

Scott N S, Shah V C, Smillie R M

出版信息

J Cell Biol. 1968 Jul;38(1):151-7. doi: 10.1083/jcb.38.1.151.

DOI:10.1083/jcb.38.1.151
PMID:5675411
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2107466/
Abstract

Chloroplasts isolated from Euglena gracilis incorporated both tritiated thymidine 5'-triphosphate and tritiated deoxyadenosine 5'-triphosphate into an acid-stable fraction. The incorporation was dependent on the presence of all four deoxynucleoside triphosphates and was sensitive to treatment with deoxyribonuclease and actinomycin D. It was demonstrated that bacterial contamination could not account for the incorporation of label. Extraction of DNA from the chloroplasts and subsequent density gradient centrifugation of the DNA in CsCl(2) showed that the incorporation was into chloroplast DNA (rho = 1.686) of high molecular weight.

摘要

从纤细裸藻分离出的叶绿体将氚标记的胸腺嘧啶核苷5'-三磷酸和氚标记的脱氧腺苷5'-三磷酸都掺入到酸稳定部分。这种掺入依赖于所有四种脱氧核苷三磷酸的存在，并且对脱氧核糖核酸酶和放线菌素D的处理敏感。已证明细菌污染不能解释标记的掺入。从叶绿体中提取DNA，随后在CsCl₂中对DNA进行密度梯度离心，结果表明掺入的是高分子量的叶绿体DNA（ρ = 1.686）。

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THE PROTEIN-SYNTHESIZING SYSTEMS FROM THE CYTOPLASM AND THE CHLOROPLASTS OF EUGLENA GRACILIS.纤细裸藻细胞质和叶绿体的蛋白质合成系统
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Evidence for the direction of chloroplasts ribosomal RNA synthesis by chloroplast DNA.叶绿体DNA指导叶绿体核糖体RNA合成方向的证据。
Biochem Biophys Res Commun. 1967 Aug 23;28(4):598-603. doi: 10.1016/0006-291x(67)90355-5.
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DNA synthesis in isolated chloroplasts.分离叶绿体中的DNA合成。
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Biosynthesis of DNA by isolated mitochondria: incorporation of thymidine triphosphate-2-C-14.分离的线粒体对DNA的生物合成：三磷酸胸苷-2-C-14的掺入
Science. 1967 Jan 6;155(3758):91-3. doi: 10.1126/science.155.3758.91.
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The synthesis of cytoplasmic DNA in synchronized Euglena.同步培养的眼虫中细胞质DNA的合成
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