Lian J B, Friedman P A
J Biol Chem. 1978 Oct 10;253(19):6623-6.
Microsomes prepared from embryonic chick bone contain a vitamin K-dependent carboxylating system which post-translationally converts glutamic acid residues in peptides to gamma-carboxyglutamic acid (gamma-CGlu). Glutamic acid residues in both endogenous chick bone microsomal protein and in the synthetic peptide Phe Leu-Glu-Glu-Val are gamma-carboxylated. These data suggest that bone cells have the capacity for de novo gamma-CGlu synthesis and may be responsible for synthesis of osteocalcin, the major gamma-CGlu protein in bone.
从胚胎期鸡骨制备的微粒体含有一种维生素K依赖性羧化系统，该系统在翻译后将肽中的谷氨酸残基转化为γ-羧基谷氨酸（γ-CGlu）。内源性鸡骨微粒体蛋白和合成肽苯丙氨酸-亮氨酸-谷氨酸-谷氨酸-缬氨酸中的谷氨酸残基都发生了γ-羧化。这些数据表明，骨细胞具有从头合成γ-CGlu的能力，可能负责骨中主要的γ-CGlu蛋白——骨钙素的合成。
