Bukrinskaia A G, Vurkunova N K, Vorkunova G K
Vopr Virusol. 1978 May-Jun(3):267-75.
At 1--3 hours after infection of chick fibroblasts and a continuous dog kidney cell line MDCK with WSN and FPV viruses large virus specific structures were found containing parent nucleocapsids, newly synthesized virus-specific RNA and newly synthesized protein. The buoyant density of these structures in cesium chloride was 1.30--1.32 g/ml. The amount of newly synthesized RNA and protein in these structures increased linearly for 3 hours of infection. The parent and newly synthesized RNA in the structures were resistant to ribonuclease. When protein synthesis was inhibited by cycloheximide, parent nucleocapsids were also found in the large structures, and primary transcription of the viral genome occurred there as well. Some structures were destroyed upon sonication of the nuclei. It is suggested that in the observed structure the parent nucleocapsids are associated with cell components (possibly, nuclear chromatin), and centers of influenza virus reproduction arise in the sites of association.
用WSN病毒和FPV病毒感染鸡胚成纤维细胞以及连续传代的犬肾细胞系MDCK 1至3小时后，发现了大型病毒特异性结构，其中包含亲代核衣壳、新合成的病毒特异性RNA和新合成的蛋白质。这些结构在氯化铯中的浮力密度为1.30至1.32克/毫升。在感染的3小时内，这些结构中新合成的RNA和蛋白质的量呈线性增加。结构中的亲代RNA和新合成的RNA对核糖核酸酶具有抗性。当用环己酰亚胺抑制蛋白质合成时，在大型结构中也发现了亲代核衣壳，并且病毒基因组的初级转录也在那里发生。对细胞核进行超声处理后，一些结构被破坏。有人认为，在所观察到的结构中，亲代核衣壳与细胞成分（可能是核染色质）相关联，并且流感病毒复制中心在关联部位形成。
