球形红假单胞菌核糖体核糖核酸中的16S和23S组分。
16s and 23s components in the ribosomal ribonucleic acid of Rhodopseudomonas spheroides.
作者信息
Szilágyi J F
出版信息
Biochem J. 1968 Sep;109(2):191-5. doi: 10.1042/bj1090191.
Abstract
- Ribosomal RNA was extracted from lysates of Rhodopseudomonas spheroides without prior isolation of ribosomes. 2. The composition of this RNA was investigated by using gradient centrifugation, showing that the proportion present as 23s component depended on the method of extraction. 3. The highest proportion of 23s component was found when cells were disrupted by ultrasonic treatment in the presence of ribonuclease inhibitors. 4. The results indicated that a ribonuclease is active in the cell lysate; this could account for the previous report (Lessie, 1965) that ribosomes of Rhodopseudomonas spheroides do not contain a 23s component.
摘要
- 不预先分离核糖体，从球形红假单胞菌的裂解物中提取核糖体RNA。2. 通过梯度离心研究了这种RNA的组成，结果表明以23s组分形式存在的比例取决于提取方法。3. 当在存在核糖核酸酶抑制剂的情况下通过超声处理破坏细胞时，发现23s组分的比例最高。4. 结果表明，细胞裂解物中有核糖核酸酶活性；这可以解释之前的报道（莱西，1965年），即球形红假单胞菌的核糖体不含有23s组分。
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