Safonova T B, Afanas'eva T I, Kravchenko N A, Cherkasov I A, Sobolev V R
Antibiotiki. 1978 Sep;23(9):797-802.
The results of the study of the effect of various concentrations of egg lysozyme on M. luteus and M. varians using 2 methods, i.e. serial dilutions in agar and turbidimetric are presented. It was found that the MIC of lysozyme for M. luteus ranged within wide limits, from less than 0.0003 to 1 mg/ml. M. varians was stable to lysozyme. The MIC for all the strains was 8 mg/ml. The turbidimetric method provided determination of general regularities in changes of the optical density in all the strains of M. luteus under the effect of various concentrations of lysozyme. On the basis of these data it was possible to consider the method as the most deep means for determining the intraspecies similarities in the surface structures of Micrococcus as compared to the method of serial dilutions in agar. The dynamics of the changes in the optical density of the M. luteus suspension markedly differed from that of M. varians.
本文展示了采用琼脂连续稀释法和比浊法这两种方法，研究不同浓度的卵溶菌酶对藤黄微球菌和变异微球菌作用的结果。结果发现，溶菌酶对藤黄微球菌的最低抑菌浓度（MIC）范围很宽，从小于0.0003至1毫克/毫升不等。变异微球菌对溶菌酶具有抗性。所有菌株的最低抑菌浓度均为8毫克/毫升。比浊法能够测定不同浓度溶菌酶作用下，藤黄微球菌所有菌株光密度变化的一般规律。基于这些数据，与琼脂连续稀释法相比，可认为该方法是确定微球菌表面结构种内相似性的最深入手段。藤黄微球菌悬浮液光密度的变化动态与变异微球菌明显不同。
