[测定微球菌和葡萄球菌对鸡蛋溶菌酶敏感性的点滴法]

[Drop method of determining micrococcal and staphylococcal sensitivity to egg lysozyme].

作者信息

Safonova T B, Givental' N I, Afanas'eva T I, Solov'eva V E, Sobolev V R

出版信息

Antibiotiki. 1978 Oct;23(10):886-8.

Abstract

Two methods for determination of staphylococcal and micrococcal sensitivity to lysozyme, i.e. the method of serial dilutions in agar and the drop method developed by the authors were compared. The drop method is a modification of the procedure described by Kloos et al. Close correlation between these two methods (r = 0.97 +/- 0.018) was found. The regression curve providing determination of the lysozyme MIC with the drop method was plotted. The drop method is more simple and economical as compared to the method of serial dilutions in agar. It has an advantage in testing sensitivity of single strains.

摘要

比较了两种测定葡萄球菌和微球菌对溶菌酶敏感性的方法，即琼脂连续稀释法和作者开发的点滴法。点滴法是对Kloos等人描述的方法的改进。发现这两种方法之间具有密切相关性（r = 0.97±0.018）。绘制了用点滴法测定溶菌酶最低抑菌浓度（MIC）的回归曲线。与琼脂连续稀释法相比，点滴法更简单、更经济。在测试单菌株敏感性方面具有优势。