从美国毒蝇伞中进行β-鹅膏毒肽的新型实验室规模纯化。

New laboratory scale purification of beta-amanitin from American Amanita phalloides.

作者信息

Yocum R R

出版信息

Biochemistry. 1978 Sep 5;17(18):3786-9. doi: 10.1021/bi00611a018.

Abstract

A modern laboratory scale method for purifying beta-amanitin from the mushroom Amanita phalloides is described. The purification involves solubilizing the toxin from dried mushrooms, removing the lipids by chloroform extraction, desalting on Amberlite XAD-4, adsorption chromatography on Sephadex LH-20 at acidic and neutral pH, and ion-exchange chromatography on QAE-Sephadex. The resulting beta-amanitin is homogeneous by thin-layer chromatography and is biologically active.

摘要

描述了一种从毒蝇伞中纯化β-鹅膏毒素的现代实验室规模方法。纯化过程包括从干蘑菇中溶解毒素、用氯仿萃取去除脂质、在Amberlite XAD-4上脱盐、在酸性和中性pH条件下在Sephadex LH-20上进行吸附色谱以及在QAE-葡聚糖凝胶上进行离子交换色谱。所得的β-鹅膏毒素通过薄层色谱分析是均一的且具有生物活性。