Saari J C, Futterman S, Stubbs G W, Heffernan J T, Bredberg L, Chan D Y, Albert D M
Invest Ophthalmol Vis Sci. 1978 Oct;17(10):988-92.
Extracts prepared from several lines of transformed cells were examined for the presence of cellular binding proteins specific for retinoids. Extracts of human retinoblastoma cell line WERI-Rb1 contained a cellular binding protein specific for retinoic acid, whereas extracts of human retinoblastoma cell line Y-79 contained cellular binding proteins for both retinol and retinoic acid. Upon purification, the latter two binding proteins proved to have properties similar to those of the corresponding proteins obtained from bovine retina. Smaller amounts of these binding proteins were detected in extracts of undifferentiated and differentiated neuroblastoma and McCoy cells. HeLa and rat glioma cells had no detectable amount of binding proteins. The 11-cis-retinal-binding protein, present in extracts of human, rat, and bovine retina, was not found in any of the cell lines examined.
检测了从几株转化细胞系制备的提取物中是否存在类视黄醇特异性细胞结合蛋白。人视网膜母细胞瘤细胞系WERI-Rb1的提取物含有视黄酸特异性细胞结合蛋白，而人视网膜母细胞瘤细胞系Y-79的提取物含有视黄醇和视黄酸的细胞结合蛋白。纯化后，后两种结合蛋白的性质被证明与从牛视网膜获得的相应蛋白相似。在未分化和分化的神经母细胞瘤及McCoy细胞的提取物中检测到较少量的这些结合蛋白。HeLa细胞和大鼠胶质瘤细胞中未检测到结合蛋白。人、大鼠和牛视网膜提取物中存在的11-顺式视黄醛结合蛋白在任何检测的细胞系中均未发现。
